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ATCC AM6-Hepatitis B virus; purified plasmid DNA
Supplier: ATCC 45020D
Full length hepatitis B virus genomic DNA in pBR322 plasmid. Purified plasmid DNA (200 ng, dried). Restriction digests of the clone give the following sizes (kb): BamHI--4.4,1.85, 1.35; EcoRI--7.5; BglII/PstI--5.2, 1.35, 0.96. AM6 is available as ATCC 39630, 40101, 45020 and 45020D. - ATCC staff The virus was isolated from plasma (subtype adw) from an HBsAg-positive donor. DNA was extracted from purified Dane particles. - personal communication AM6 is available at ATCC 39630, ATCC 40101, and ATCC 45020. The 1350 bp BamHI fragment encodes both group and subtype-specific determinants of HBsAg. The complete viral genome was linearized with BamHI and inserted into pBR322. - U.S. Pat. 4,942,125 dated July 17, 1990 A BamHI digest cuts the insert into fragments of 1.85 kb and 1.35 kb. The 1.35 kb fragment contains coding sequences for HBsAg and most of region P. The 1.85 kb fragment encodes HBcAg and most of the X gene product. - U.S. Pat. 4,942,125 dated July 17, 1990 The
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