Invitrogen™ TBE (10X), RNase-free

Catalog No.	Quantity
AM9863	1 L
AM9865	4 x 1 L

Catalog No. AM9863 Supplier Invitrogen™ Supplier No. AM9863

Description

Molecular biology grade, Invitrogen 10X TBE solution is supplied in four bottles containing 1 L each. The buffer is certified RNase-free, economical, and ready-to-use.

Molecular biology grade, Invitrogen 10X TBE solution is supplied in four bottles containing 1 L each. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Invitrogen's nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.

Order Info

Shipping Condition: Room temperature

Certifications

Certified RNase-free

Specifications

• Chemical Name or Material: Running Buffer
• Concentration: 10X
• Product Line: Ambion
• Quantity: 1 L
• Grade: Molecular Biology

Frequently Asked Questions (FAQs)

Can I autoclave agarose to prepare agarose gels?

No. Autoclaving at temperatures that are too high or pressures that are too high can hydrolyze the agarose polymer and weaken it.

Do you have any tips for preparing high-percentage (3% to 4%) agarose gels?

Add the powder to cold buffer while stirring. Let the agarose rehydrate for 1 to 2 hr and then heat slowly until agarose is completely dissolved. Using a microwave at low power settings is acceptable.

What can I do to prevent static on agarose bottles?

One recommendation: get a supply of fabric softener sheets and wipe the bottle with a sheet before opening it.

How can I avoid the build-up of the precipitous film sometimes seen in 10X TBE?

The precipitation of concentrated TBE stocks may be due to nucleation of salt crystals by dust particles or other insoluble materials. Therefore, filtering the solution through a 0.2 µm cellulose acetate or cellulose nitrate filter after preparation helps avoid this precipitate. [Mayeda A, Krainer A (1991) BioTechniques 10.2, 1820]

How can generation of replication competent adenoviruses be avoided when using your pSilencer adeno 1.0-CMV System?

Although there is only a very small chance of creating replication competent virus, steps should still be taken to avoid added risk. The virus is expanded in two rounds of amplification, and all secondary expansions should be performed from an initial expansion stock. Secondary amplifications in HEK293 cells should not be performed from the stock of another secondary expansion, as this could increase the chance of making replication competent virus. See the product manual for more detail and guidelines for screening.

For Research Use Only. Not for use in diagnostic procedures.