Invitrogen™ 10X TBE (powder)

Catalog No.	Quantity
AM9864	10 x 1 L

Catalog No. AM9864 Supplier Invitrogen™ Supplier No. AM9864

Description

Invitrogen 10X TBE (Tris-Borate-EDTA Buffer) is thoroughly tested for RNase and DNase contamination, making it ideal for use in sensitive application.

Invitrogen 10X TBE (Tris-Borate-EDTA Buffer) is thoroughly tested for RNase and DNase contamination, making it ideal for use in sensitive application. 10 packets are provided, each containing the necessary powder to make 1 L of 10X TBE buffer upon the addition of H₂O. These reagents are rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.

Order Info

Shipping Condition: Room temperature

Specifications

• Chemical Name or Material: Running Buffer
• Concentration: 10X
• For Use With (Application): Nucleic acid gel electrophoresis & blotting
• Physical Form: Powder
• Product Line: Ambion
• Quantity: 10 x 1 L

Frequently Asked Questions (FAQs)

Can I autoclave agarose to prepare agarose gels?

No. Autoclaving at temperatures that are too high or pressures that are too high can hydrolyze the agarose polymer and weaken it.

Do you have any tips for preparing high-percentage (3% to 4%) agarose gels?

Add the powder to cold buffer while stirring. Let the agarose rehydrate for 1 to 2 hr and then heat slowly until agarose is completely dissolved. Using a microwave at low power settings is acceptable.

What can I do to prevent static on agarose bottles?

One recommendation: get a supply of fabric softener sheets and wipe the bottle with a sheet before opening it.

How can I avoid the build-up of the precipitous film sometimes seen in 10X TBE?

The precipitation of concentrated TBE stocks may be due to nucleation of salt crystals by dust particles or other insoluble materials. Therefore, filtering the solution through a 0.2 µm cellulose acetate or cellulose nitrate filter after preparation helps avoid this precipitate. [Mayeda A, Krainer A (1991) BioTechniques 10.2, 1820]

How can generation of replication competent adenoviruses be avoided when using your pSilencer adeno 1.0-CMV System?

Although there is only a very small chance of creating replication competent virus, steps should still be taken to avoid added risk. The virus is expanded in two rounds of amplification, and all secondary expansions should be performed from an initial expansion stock. Secondary amplifications in HEK293 cells should not be performed from the stock of another secondary expansion, as this could increase the chance of making replication competent virus. See the product manual for more detail and guidelines for screening.

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.