Invitrogen mirVana miRNA Isolation Kit, with phenol
Available with and without phenol
Manufacturer: Invitrogen AM1560
The mirVana miRNA Isolation Kit uses a rapid procedure to isolate small RNAs from tissue and cells using an efficient glass fiber filter (GFF)-based method. The method isolates total RNA ranging in size from kilobases down to 10-mers. Each kit contains sufficient reagents and consumables for either 40 isolations of total RNA (including small RNAs), or 20 separate large and small RNA fractions.
Efficient recovery of small RNA Current RNA purification procedures rely on organic extraction, followed by alcohol precipitation or adsorption of nucleic acid molecules on a GFF or silicate matrix. Unfortunately, the former procedure requires alcohol precipitation, which is time consuming and inefficiently recovers small RNAs. The latter procedure typically results in the loss of substantial amounts of small RNA. The mirVana miRNA Isolation Kit uses organic extraction followed by purification on a GFF under specialized binding and wash conditions. As a result, the kit effectively recovers all RNAfrom large mRNA and ribosomal RNA down to 10-mersfrom virtually all cell and tissue types. The mirVana miRNA Isolation Kit enables the isolation of small RNA-containing total RNA from samples consisting of 102107 cultured cells or 0.5250 mg tissue.
Enrich for small RNA Although the mirVana miRNA Isolation Kit efficiently purifies all RNA larger than 10nt, it also includes procedures for isolating RNA fractions, specifically enriched or depleted in small RNA species. Through the enrichment procedure included in the mirVana miRNA Isolation Kit, RNA molecules of ~200 nt and less can be efficiently purified away from the larger RNA species. The resulting RNA preparation is highly enriched for miRNAs, siRNAs, and/or snRNAs. Small RNA enrichment using the mirVana miRNA Isolation Kit procedure allows more sensitive small RNA detection with less background as compared to the same assay used with total RNA.
- Efficient isolation of small RNA-containing total RNA
- Enrich for small RNA (<200nt) to increase sensitivity in downstream analyses
- Simple 30 minute procedure
- Ideal for miRNA, siRNA, shRNA, and snRNA analysis
- Compatible with virtually all cell and tissue types
Accessory products For tissues that have very high levels of ribonucleases, such as pancreas or spleen, the mirVana miRNA Isolation Kit is recommended, although it is not designed to recover protein and small RNA from the same sample.
Not high-throughput compatible (Manual)
DNA and RNA Purification and Analysis, RNA Extraction, RNAi, RNAi, Epigenetics and Non-Coding RNA Research, miRNA and Non-Coding RNA Analysis, miRNA Isolation, microRNA Isolation, siRNA
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|Total RNA, Transcriptome RNA, siRNA, snRNA, micro RNA|
|30mL miRNA Wash Solution I; 50mL Wash Solution 2/3; 80 Collection Tubes; 40 Filter Cartridges; 100mL Lysis/Binding Buffer; 10mL miRNA Homogenate Additive; 100mL Acid-Phenol:Chloroform; 1.4mL Gel Loading Buffer II; 5mL Elution Solution|
|• 30 ml miRNA Wash Solution I (room temperature)
• 50 ml Wash Solution 2/3 (room temperature)
• 80 Collection Tubes (room temperature)
• 40 Filter Cartridges (room temperature)
• 100 ml Lysis/Binding Buffer (4°C)
• 10 ml miRNA Homogenate Additive (4°C)
• 100 ml Acid-Phenol:Chloroform (4°C)
• 1.4 ml Gel Loading Buffer II (-20°C)
• 5 ml Elution Solution (-20°C, 4°C, or room temperature)
|Up to 250 mg tissue, Up to 10^7 cells|
|Plant Samples, Viral Samples, Tissue, Yeast, Bacteria, Cells|
|Spin Column (Glass Fiber Filter), Organic Extraction|
|Not High-throughput Compatible (Manual)|
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