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Invitrogen™ MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit, compatible with PAXgene™ Blood RNA Tubes Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Magnetic bead-based technology to purify total RNA

Manufacturer:  Invitrogen™ 4451894

Catalog No. 44-518-94


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Includes: Kit Components Box 1: 1 PAXgene™ Resuspension Solution, 18mL; Wash Solution 1 Concentrate, 16mL (Before using the Wash Solution 1 Concentrate, add the correct volume of isopropanol (user-supplied), as indicated on the bottle); Wash Solution 2 Concentrate, 2 x 30mL (Before using the Wash Solution 2 Concentrate, add the correct volume of ethanol (user-supplied), as indicated on the bottle); RNA Rebinding Buffer 10mL; Elution Buffer 10mL; Processing Plate (96-well) 1 plate; 2.0mL Tubes 100 tubes, RNA Binding Beads, 2mL. Box 2: 2 PAXgeneR Proteinase, 0.6mL; 10x TURBO™ DNase Buffer, 1mL; TURBO™ DNase, 1mL

Description

Description

The MagMAX for Stabilized Blood Tubes RNA Isolation Kit purifies total RNA (including miRNA) from PAXgene™ Blood RNA Tubes using proven magnetic bead-based technology. A TURBO DNase™ step efficiently eliminates contaminating gDNA ensuring the highest purity RNA possible suitable for real-time RT-PCR and microarray analysis. Maximize Total RNA Yields From Blood Samples The MagMAX for Stabilized Blood Tubes RNA Isolation Kit utilizes proven magnetic bead-based technology to purify maximal yields of total RNA from PAXgene Blood RNA Tubes. The MagMAX for Stabilized Blood Tubes RNA Isolation Kit can recover 3-18 µg from a single PAXgene Blood RNA tube. A TURBO DNase step efficiently eliminates contaminating gDNA that can interfere with downstream applications and ensures the highest purity RNA possible. The MagMAX for Stabilized Blood Tubes RNA Isolation Kit performs exceptionally well at purifying miRNA with total RNA from PAXgene Blood RNA Tubes without having to employ a separate miRNA protocol. One protocol does it all. The MagMAX for Stabilized Blood Tubes RNA Isolation Kit can also detect miRNA from PAXgene Blood RNA tubes as shown by TaqMan™ gene expression assays. Users are strongly advised to perform appropriate validation studies against their miRNA targets. Rapid Processing Time Processing times will vary based on format selected, but twelve PAXgene Blood RNA tubes can be processed manually with the MagMAX for Stabilized Blood Tubes RNA Isolation Kit in approximately 2 hours. An Alternative to Full Purification For users who perform primarily TaqMan-based real-time RT-PCR experiments, and do not need to archive RNA/cDNA, an alternative to the MagMAX for Stabilized Blood Tubes RNA Isolation Kit is the Stabilized Blood-to-CT Nucleic Acid Preparation Kit for qPCR. This kit makes it possible to perform expression analysis directly from PAXgene Blood RNA tubes without conventional RNA purification. One-Stop Shopping MagMAX for Stabilized Blood Tubes RNA Isolation Kit is compatible with reverse transcription mixes (SuperScript™), PCR master mixes (both TaqMan and SYBRbased), and other blood workflow products.

  • Flexible purification format—use microfuge tubes, manual 96-well plates, or open-format automated 96-well plates from the same kit
  • Simple magnetic bead workflow—no spin columns
  • Total RNA without RNase activity, contaminating gDNA, or RT-PCR inhibitors
  • Suitable for use with automated protocols, high-throughput compatible

Flexible Purification Formats

  • The MagMAX for Stabilized Blood Tubes RNA Isolation Kit can be processed in low-, medium- or high-throughput formats with superior workflow and handling compared to traditional competitive methods
  • For low-throughput users, the MagMAX for Stabilized Blood Tubes RNA Isolation Kit can be used in individual 1.5mL microfuge tubes with the bead-RNA-complex remaining in a single processing tube until the final elution step
  • For medium-to-high–throughput users, the MagMAX for Stabilized Blood Tubes RNA Isolation Kit can be done in a 96-well processing plate with either a single or multi-channel pipettor to accelerate the purification process
  • For high-throughput users, the MagMAX for Stabilized Blood Tubes RNA Isolation Kit is compatible with manual and automated 96-well processing
  • Simply process multiple batches of tubes in series or in parallel, through the up-front centrifugation process, to the convenient stopping point, until enough samples can be loaded onto a 96-well processing plate for the RNA purification steps
  • The RNA purification process can be handled on either open-format liquid handlers, in conjunction with a 96-well magnetic stand on the deck, or magnetic bead movers like the MagMAX Express-96 Magnetic Particle Processor (deep well version only)
Specifications:
  • Yield (Range): 3 - 18µg
  • Quality (RIN) ): ≥ 8
  • Quality (A260/280) ): 1.8 – 2.2
  • gDNA (w/w) ): <1.56%
  • Number of Preps: 96 preparations (blood tubes)

DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Blood

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Specifications

Specifications

Total RNA
Total RNA
Tube(s), 96-well plate
18 µg
High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
Ambion™, MagMAX™
Real-Time Quantitative PCR (qPCR)
Blood
Magnetic Bead
Kit Components
Box 1:
Store at RT
• 1 PAXgene® Resuspension Solution, 18 mL
• Wash Solution 1 Concentrate, 16 mL (Before using the Wash Solution 1 Concentrate, add the correct volume of isopropanol (user-supplied), as indicated on the bottle)
• Wash Solution 2 Concentrate, 2 x 30 mL (Before using the Wash Solution 2 Concentrate, add the correct volume of ethanol (user-supplied), as indicated on the bottle)

• RNA Rebinding Buffer 10 mL
• Elution Buffer 10 mL
• Processing Plate (96-well) 1 plate
• 2.0 mL Tubes 100 tubes

Store at 2 to 8 °C, Do not freeze
• RNA Binding Beads, 2 mL

Box 2:
Store at -15 to -25 °C
• 2 PAXgeneR Proteinase, 0.6 mL
• 10x TURBO™ DNase Buffer, 1 mL
• TURBO™ DNase, 1 mL
1.7 hrs
SDS
Documents
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For Research Use Only. Not for use in diagnostic procedures.