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Invitrogen™ MAXIscript™ SP6 Kit with Manual

Catalog No. AM1310
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Product Type:
SP6 In Vitro Transcription Kit
SP6 In Vitro Transcription Kit with Manual
Quantity:
100 Reactions
30 Reactions
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Catalog No. Product Type Quantity
AM1310 SP6 In Vitro Transcription Kit 100 Reactions
AM1310M SP6 In Vitro Transcription Kit with Manual 100 Reactions
AM1308 SP6 In Vitro Transcription Kit 30 Reactions
AM1308M SP6 In Vitro Transcription Kit with Manual 30 Reactions
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Catalog No. AM1310 Supplier Invitrogen™ Supplier No. AM1310
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Includes

SP6 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, pTRI-b-actin, Nuclease-free Water

Fast synthesis of RNA probes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations

Ambion MAXIscript SP6 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations. Each kit contains sufficient reagents for 30 reactions.

  • High yields of high specific activity, full-length RNA probes in just 10 minutes (RNA Yield: 2-6μg)
  • Ideal probe synthesis kit for nuclease protection assays, Northern and Southern blots, and in situ hybridization
  • Includes TURBO DNase
    Contain quality-tested DNase/RNase-free reagents
  • Synthesizes RNA probes with specific activities reaching 1 x 109 cpm/μg in just 10 minutes
  • Efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them perfect companion to ribonuclease protection assays
  • For synthesis of nonisotopically labeled probes
  • Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar™ Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into reaction
  • For synthesis of modest amounts of unlabeled RNA (2–6μg/20μL reaction) of up to 2kb

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage SP6 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Final Product Type Synthetic mRNA, mRNA
Format Kit
Sample Type mRNA, Synthetic mRNA
For Use With (Application) In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
No. of Reactions 100 Reactions
Product Line Ambion, MAXIscript
Product Type SP6 In Vitro Transcription Kit
Quantity 100 Reactions
Promoter SP6
Shipping Condition Dry Ice
Yield 2-6 μg
Starting Material PCR Products, Linearized DNA plasmid
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For Research Use Only. Not for use in diagnostic procedures.

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