Invitrogen™ MEGAclear™ Transcription Clean-Up Kit
Quickly cleanup large-scale transcription reactions
Supplier: Invitrogen™ AM1908
DescriptionMEGAclear Kit is designed for quick cleanup of any large-scale transcription reaction. MEGAclear Kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components.
- Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components
High recovery from 1ng to 500μg of input
Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections
Quick protocol—purify RNA in less than 30 minutes
- Using the MEGAclear Kit: Protocol is quick and easy. Transcription reaction is mixed with Binding Solution, passed over an RNA-binding filter, and washed; an elution step releases the purified RNA, which is ready for any application requiring highly pure synthetic or in vitro-transcribed RNA. For RNA recovery of 70% or greater, single-stranded RNA should be >100 nt and double-stranded RNA should be >200 bp.
|Binding Solution, Wash Solution Concentrate, and 5 M Ammonium Acetate are stored at 4°C. Elution Solution can be stored at any temperature, and Filter Cartridges and Collection and Elution Tubes are stored at room temperature.|
|mRNA, Synthetic mRNA|
|In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products|
|Not High-throughput Compatible (Manual)|
|Purified Synthetic mRNA, Purified mRNA|
|Silica Spin Column|
|mRNA, Synthetic mRNA|
|Vacuum Manifold, Centrifuge|
|mRNA purification kit, Purification kit|
|1 ng to 500 µg of dsRNA>200b or ssRA>100bp|
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For Research Use Only. Not for use in diagnostic procedures.