Invitrogen™ MEGAshortscript™ T7 Transcription Kit

Description

Maximize the yield of short RNA transcripts

The MEGAshortscript T7 Kit is designed for high yields of in vitro-transcribed RNA products, in the 20 to 500 nucleotide range. Using conventional methods, transcription of small templates often results in low mass yields of RNA. Short transcripts require many more transcription initiation events to synthesize a given mass of product. Since the initiation step is the rate-limiting step in transcription reactions, the yield of product from short templates is often low. The MEGAshortscript T7 Kit has been designed to overcome the drawbacks involved in conventional in vitro transcription to maximize the yield of short RNA transcripts.

• Efficiently transcribes small templates (<300 bases) with consistently high yields
• Designed for use with plasmid, synthetic oligonucleotide, or PCR product templates

Specifications

• Content And Storage: T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-RNA 18S, TURBO DNase, Ammonium Acetate Stop Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
• Final Product Type: Synthetic mRNA, mRNA
• Format: Kit
• Sample Type: mRNA, Synthetic mRNA
• For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
• No. of Reactions: 25 Reactions
• Product Line: Ambion™, MEGAshortscript™
• Product Type: T7 In Vitro Transcription kit
• Quantity: 25 Reactions
• Promoter: T7
• Shipping Condition: Dry Ice
• Starting Material: PCR Products, Linearized DNA plasmid

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.