Invitrogen mMESSAGE mMACHINE SP6 Transcription Kit
in vitro synthesis of large amounts of capped RNA
Manufacturer: Invitrogen AM1340
mMESSAGE mMACHINE kits are designed for in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5 ft. end.
- RNA Yield: 5-25µg
- Include cap analog [m7G(5 ft.)ppp(5 ft.)G] in an ultra high-yield transcription reaction
- Cap analog is incorporated only as first or 5 ft. terminal G of transcript because its structure precludes its incorporation at other position in RNA molecule
- Simplified reaction format in which all four ribonucleotides and cap analog are mixed in single solution
- Ideal for routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications
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|In Vitro Transcribed RNA, Capped RNA|
|Ambion™, mMESSAGE mMACHINE®|
|SP6 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.|
|PCR Products, Linearized DNA|
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