Invitrogen™ mMESSAGE mMACHINE™ SP6 Transcription Kit

Description

in vitro synthesis of large amounts of capped RNA

mMESSAGE mMACHINE™ kits are designed for in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5′ end.

• RNA Yield: 5-25μg
• Include cap analog [m7G(5′)ppp(5′)G] in an ultra high-yield transcription reaction
• Cap analog is incorporated only as first or 5′ terminal G of transcript because its structure precludes its incorporation at other position in RNA molecule
• Simplified reaction format in which all four ribonucleotides and cap analog are mixed in single solution
• Ideal for routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications

Specifications

• Content And Storage: SP6 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
• Final Product Type: Synthetic mRNA, mRNA
• Format: Kit
• Sample Type: mRNA, Synthetic mRNA
• For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
• No. of Reactions: 25 Reactions
• Product Line: Ambion™, mMESSAGE mMACHINE™
• Product Type: SP6 In Vitro Transcription kit
• Quantity: 25 Reactions
• Promoter: SP6
• Shipping Condition: Dry Ice
• Yield: 15-25 μg
• Starting Material: PCR Products, Linearized DNA plasmid

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.