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Invitrogen™ mMESSAGE mMACHINE™ SP6 Transcription Kit
in vitro synthesis of large amounts of capped RNA
Manufacturer: Invitrogen™ AM1340
Description
mMESSAGE mMACHINE™ kits are designed for in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5′ end.
- RNA Yield: 5-25μg
- Include cap analog [m7G(5′)ppp(5′)G] in an ultra high-yield transcription reaction
- Cap analog is incorporated only as first or 5′ terminal G of transcript because its structure precludes its incorporation at other position in RNA molecule
- Simplified reaction format in which all four ribonucleotides and cap analog are mixed in single solution
- Ideal for routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications
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Specifications
| SP6 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature. | |
| Kit | |
| In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products | |
| Ambion™, mMESSAGE mMACHINE™ | |
| 25 Reactions | |
| Dry Ice | |
| PCR Products, Linearized DNA plasmid |
| synthetic mRNA, mRNA | |
| mRNA, synthetic mRNA | |
| 25 Reactions | |
| SP6 In Vitro Transcription kit | |
| SP6 | |
| 15-25 µg |
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