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Invitrogen™ mMESSAGE mMACHINE™ SP6 Transcription Kit Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

in vitro synthesis of large amounts of capped RNA

Manufacturer:  Invitrogen™ AM1340

Catalog No. AM1340

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mMESSAGE mMACHINE™ kits are designed for in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5 ft. end.

  • RNA Yield: 5-25µg
  • Include cap analog [m7G(5 ft.)ppp(5 ft.)G] in an ultra high-yield transcription reaction
  • Cap analog is incorporated only as first or 5 ft. terminal G of transcript because its structure precludes its incorporation at other position in RNA molecule
  • Simplified reaction format in which all four ribonucleotides and cap analog are mixed in single solution
  • Ideal for routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications

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In Vitro Transcribed RNA, Capped RNA
Dry Ice
15-25 µg
SP6 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
PCR Products, Linearized DNA
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