Invitrogen™ mMESSAGE mMACHINE™ SP6 Transcription Kit
in vitro synthesis of large amounts of capped RNA
Manufacturer: Invitrogen™ AM1340
mMESSAGE mMACHINE™ kits are designed for in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5′ end.
- RNA Yield: 5-25μg
- Include cap analog [m7G(5′)ppp(5′)G] in an ultra high-yield transcription reaction
- Cap analog is incorporated only as first or 5′ terminal G of transcript because its structure precludes its incorporation at other position in RNA molecule
- Simplified reaction format in which all four ribonucleotides and cap analog are mixed in single solution
- Ideal for routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications
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|SP6 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.|
|In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products|
|Ambion™, mMESSAGE mMACHINE™|
|PCR Products, Linearized DNA plasmid|
|synthetic mRNA, mRNA|
|mRNA, synthetic mRNA|
|SP6 In Vitro Transcription kit|
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