Invitrogen™ mMESSAGE mMACHINE™ SP6 Transcription Kit  

in vitro synthesis of large amounts of capped RNA

Manufacturer:  Invitrogen™ AM1340

Catalog No. AM1340

Description

mMESSAGE mMACHINE™ kits are designed for in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5 ft. end.

• RNA Yield: 5-25µg
• Include cap analog [m7G(5 ft.)ppp(5 ft.)G] in an ultra high-yield transcription reaction
• Cap analog is incorporated only as first or 5 ft. terminal G of transcript because its structure precludes its incorporation at other position in RNA molecule
• Simplified reaction format in which all four ribonucleotides and cap analog are mixed in single solution
• Ideal for routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications

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Specifications

• Final Product Type: In Vitro Transcribed RNA, Capped RNA
• Promoter: SP6
• Shipping Condition: Dry Ice
• Product Line: Ambion™, mMESSAGE mMACHINE®

• Yield: 15-25 µg
• Content And Storage: SP6 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
• Starting Material: PCR Products, Linearized DNA