Invitrogen™ mMESSAGE mMACHINE™ T7 Transcription Kit

Description

in vitro synthesis of large amounts of capped RNA

mMESSAGE mMACHINE™ kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5′ end.

• RNA Yield: 20-30μg
• Include cap analog [m⁷G(5′)ppp(5′)G] in ultra high-yield transcription reaction
• Simplified reaction format in which all four ribonucleotides and cap analog are mixed in single solution
• Cap analog: GTP ratio of solution is 4:1, which is optimal for maximizing both RNA yield and the proportion of capped transcripts
• For routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications
• High yields are achieved by optimizing reaction conditions for RNA synthesis in presence of high nucleotide concentrations
• RNA synthesized is protected from degradation by contaminating ribonucleases that present with RNase inhibitor - component of the Enzyme Mix

Specifications

• Content And Storage: T7 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
• Final Product Type: Synthetic mRNA, mRNA
• Format: Kit
• Sample Type: mRNA, Synthetic mRNA
• For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
• No. of Reactions: 25 Reactions
• Product Line: Ambion™, mMESSAGE mMACHINE™
• Product Type: T7 In Vitro Transcription kit
• Quantity: 25 Reactions
• Promoter: T7
• Shipping Condition: Dry Ice
• Yield: 20-30 μg
• Starting Material: PCR Products, Linearized DNA plasmid

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.