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Invitrogen™ mMESSAGE mMACHINE™ T7 Transcription Kit Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

in vitro synthesis of large amounts of capped RNA

Manufacturer:  Invitrogen™ AM1344

Catalog No. AM1344

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mMESSAGE mMACHINE™ kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5 ft. end.

  • RNA Yield: 20-30µg
  • Include cap analog [m7G(5 ft.)ppp(5 ft.)G] in ultra high-yield transcription reaction
  • Simplified reaction format in which all four ribonucleotides and cap analog are mixed in single solution
  • Cap analog: GTP ratio of solution is 4:1, which is optimal for maximizing both RNA yield and the proportion of capped transcripts
  • For routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications
  • High yields are achieved by optimizing reaction conditions for RNA synthesis in presence of high nucleotide concentrations
  • RNA synthesized is protected from degradation by contaminating ribonucleases that present with RNase inhibitor - component of the Enzyme Mix

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In Vitro Transcribed RNA, Capped RNA
Dry Ice
20-30 µg
T7 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
PCR Products, Linearized DNA
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