Invitrogen™ Mouse RiboPure™-Blood RNA Isolation Kit with Manual

Catalog No.	Quantity	Product Type
AM1951	25 preps	Kit

Catalog No. AM1951 Supplier Invitrogen™ Supplier No. AM1951

Description

Includes

Acid–Phenol:Chloroform, 3M Sodium Acetate pH 5.5, Wash Solution 1 Concentrate, Wash Solution 2/3 Concentrate, 2mL tubes pre-loaded with RNAlater, Lysis Solution, Filter Cartridges, Collection Tubes, Nuclease–free Water and Elution solution

For the purification of extremely high-quality total RNA (and optionally, microRNA) from mouse or rat blood in about 35 minutes.

For the purification of extremely high-quality total RNA (and optionally, microRNA) from mouse or rat blood in about 35 minutes. The Ambion™ kit includes sufficient reagents for 25 RNA purifications from whole blood samples ranging from 0.1–0.5 each.

• Includes RNAlater™, predispensed in 2 mL tubes, for RNA stabilization
• Resulting RNA is suitable for stringent downstream applications such as array analysis and qRT-PCR

Due to interference from the enzymatic inhibitors and anticoagulants present in blood samples, RNA purification from whole blood requires a stringent isolation method that yields high-quality RNA depleted of inhibiting agents and contaminants. The kit includes microfuge tubes preloaded with RNAlater™ Tissue Collection:RNA Stabilization Solution, an aqueous, nontoxic storage reagent that prevents RNA degradation and preserves RNA profiles within samples. Stabilized samples can be stored at room temperature for up to 3 days or at –20°C for long-term storage. The related Figures show the performance of RNA isolated with the Mouse RiboPure™-Blood Kit in real-time RT-PCR. The kit includes an alternative protocol for isolating total RNA that contains microRNA. Typically, 30–55 μg of total RNA (containing microRNA) is obtained from 0.5 mL mouse blood. Yields from rat blood are considerably more variable, generally 18–40 μg from 0.5 mL blood.

Order Info

Shipping Condition: Room temperature

Specifications

• Content And Storage: Acid-Phenol:Chloroform, 3M Sodium Acetate pH 5.5, Wash Solution 1 Concentrate, Wash Solution 2/3 Concentrate at 4°C. Store 2 mL tubes pre-loaded with RNAlater, Lysis Solution, Filter Cartridges, Collection Tubes, Nuclease-free Water and Elution solution at room temperature.
• Format: Filter Cartridge
• Isolation Technology: Organic Extraction, Spin Column
• Purification Time: 35 min.
• Species: Mouse
• Sample Type: Blood
• Final Product Type: Total RNA, micro RNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• For Use With (Application): Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
• Includes: Acid–Phenol:Chloroform, 3M sodium acetate pH 5.5, wash solution 1 concentrate, wash solution 2/3 concentrate, 2mL tubes pre-loaded with RNAlater, lysis solution, filter cartridges, collection tubes, nuclease-free water and elution solution
• Product Line: Ambion, RiboPure
• Product Type: Kit
• Quantity: 25 preps
• Shipping Condition: Room Temperature
• Starting Material Amount: Up to 0.5 ml whole blood
• Target: miRNA, Total RNA
• Yield: 40 μg

Frequently Asked Questions (FAQs)

What is the difference between the RiboPure Blood Purification Kit (Cat. No. AM1928) and Mouse RiboPure Blood RNA Isolation kit (Cat. No. AM1951)?

The former (Cat. No. AM1928) is for isolation of RNA from human blood and the latter (Cat. No. AM1951) is for isolation of RNA from mouse and rat blood. Both kits have the same columns but differ in protocol and the wash 1 buffer, wash 2/3 buffer, and sodium acetate solutions. We do not recommend using the mouse kit for human blood samples. The mouse kit accommodates the difference in viscosity of mouse/rat vs. human blood and also the higher mouse and rat blood RNA yield. The mouse kit allows recovery of either total RNA containing small RNAs or total RNA depleted of small RNAs based on which protocol in the manual was followed. With the RiboPure Blood Purification Kit, using the standard protocol in the manual, you can isolate total RNA depleted of small RNAs, but with an alternate protocol, you can isolate total RNA containing small RNAs. Alternatively, if you need small RNAs alone, then you should follow the above alternate protocol for isolation of total RNAs containing small RNAs, and then follow Section IVA in the mirVana miRNA Isolation Kit manual (Cat. No AM1560).

What is the column cut-off size for the RiboPure RNA Purification Kits?

The column cut-off size is 200 nt. Using a RiboPure kit is not appropriate for miRNA recovery. For miRNA isolation, we would suggest using a mirVana kit, the TRIzol Plus RNA Purification Kit, or the PureLink RNA Micro Kit.

What are the differences in technology between the PureLink RNA Mini Kit and RiboPure RNA Purification Kits?

The PureLink RNA Mini Kit provides rapid column-based purification of total RNA, without organic lysis (phenol/chloroform). You can obtain up to 1,000 µg of purified RNA from a single extraction. The RiboPure RNA Purification Kits combine a phenol/guanidine thiocyanate solution with a glass-fiber filter purification method.

Do Tempus Blood RNA Tubes (Cat. No. 4342792) stabilize RNA similarly to RNAlater Stabilization Solution (Cat. No. AM7021)?

Both Tempus Blood RNA Tubes and RNAlater Stabilization Solution employ the same effective stabilization of mRNA expression profiles, and eliminate the need to isolate the RNA immediately after sample collection. However, there are some key differences:
- Components in each stabilization solution are different. The exact components and differences are proprietary.
- The RNAlater Stabilization Solution preserves RNA from degradation without lysing blood cells.
- Samples in RNAlater Stabilization Solution can be safely stored for up to 1 week at 25 degrees C, or up to 1 month at 4 degrees C.

For Research Use Only. Not for use in diagnostic procedures.