Invitrogen™ mRNA Catcher™ PLUS Purification Kit

Description

Includes

1 96-well mRNA Catcher PLUS plate; 4mL 2X Lysis Buffer L20; 30mL Wash Buffer W15; 4mL Dilution Buffer DL1; 8mL Elution Buffer E3; 100μL 0.5M DTT (4°C)

The mRNA Catcher PLUS Purification Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format.

The mRNA Catcher PLUS Purification Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format. By taking advantage of locked nucleic acid (LNA) technology, the mRNA Catcher PLUS kit provides improved performance and a faster protocol, while preserving mRNA yield and purity.

The mRNA Catcher PLUS Purification Kit offers:

• Robust performance—high binding capacity per well (>80 ng/well)
• Reproducible results—low CVs from well-to-well and plate-to-plate (∼2%)
• Optimized protocols—for small sample sizes (100 cells, 4 mg tissue, and 40 μL blood)
• Compatible plate design—may be used with most PCR cyclers

How it works

mRNA from total RNA, cells, tissue, and blood samples is hybridized to an immobilized, single-stranded 20-mer oligo(dT)-containing LNA. The incorporation of LNA into the oligo probe increases the hybridization efficiency and specificity of the mRNA binding. The mRNA can either be eluted or reversed-transcribed to cDNA directly in the well. The mRNA Catcher PLUS method does not require centrifugation, precipitation, phase separation, or column filtration. In addition, the entire method is amenable to automation for streamLined, high-throughput expression profiling for large-scale processes. Isolated mRNA is suitable for use in downstream applications such as RT-PCR and qRT-PCR.

Order Info

Shipping Condition: Wet ice

Specifications

• Content And Storage: • 1 x 96-well mRNA Catcher™ PLUS Plate; store at 4°C
  • 4 mL 2X Lysis Buffer L20; room temperature
  • 30 mL Wash Buffer W15; room temperature
  • 4 mL Dilution Buffer DL1; room temperature
  • 8 mL Elution Buffer E3 (DEPC-treated RNase-free water); room temperature
  • 100 μL 0.5M DTT; 4°C
• Isolation Technology: Oligo dT Affinity
• Purification Time: 1.5 hr. (Including sample homogenization)
• Elution Volume: 80 μL
• Sample Type: Cells, Tissue, Total RNA, Whole Blood
• Final Product Type: mRNA
• High-throughput Compatibility: High-throughput Compatible
• For Use With (Equipment): Robotic Liquid Handling Systems
• For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, Microarray Analysis
• Product Type: Purification Kit
• Quantity: 96 Preps
• Shipping Condition: Room Temperature
• Starting Material Amount: Cells: 100–10⁶, Tissue: 4–40 mg, Total RNA: 0.1–100 μg, Whole Blood: 40 μL
• Yield: >80 ng

For Research Use Only. Not for use in diagnostic procedures.