Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

Catalog No.	Quantity
AM9937	10 x 50 mL
AM9938	1 x 100 mL
AM9930	1 x 500 mL
AM9939	5 x 100 mL
AM9932	1 x 1000 mL
43-879-36	4 x 1000 mL

Catalog No. AM9937 Supplier Invitrogen™ Supplier No. AM9937

Description

Includes

10 x 50 mL bottles.

Nuclease-free Water (not DEPC-treated) has been deionized, filtered into the final bottle, and autoclaved. It is ready to use and requires no preparation, mixing, or autoclaving.

Nuclease-free, non-DEPC-treated water for NGS, PCR, qPCR, dPCR, and RNA/DNA workflows, delivering verified purity and reliable performance. This molecular biology grade water is produced through controlled manufacturing and multi-step purification to achieve high purity and consistent performance. Each lot undergoes functional testing to confirm the absence of RNase, exonuclease, and nonspecific endonuclease activity, ensuring nucleic acid integrity during preparation, storage, and downstream applications. Manufactured in the USA under ISO 9001 and ISO 13485 certified quality systems, it is supported by comprehensive quality control documentation for full traceability and reproducibility.

• Tested for nuclease activity – Each lot is tested for RNase, exonuclease, and endonuclease activity using validated methods to ensure suitability for sensitive molecular biology applications
• Non-DEPC treated – Eliminates the risk of residual Diethyl pyrocarbonate (DEPC) and by-products that can inhibit DNA polymerase, ensuring reliable PCR performance and making it suitable for sensitive applications such as oocyte microinjection
• Optimized for qPCR and sequencing workflows – Ideal for PCR, qPCR, dPCR, next-generation sequencing, and nucleic acid storage

Advanced Manufacturing and Quality Control

Manufactured in Austin, TX, USA under ISO 9001 and ISO 13485 certified quality systems, this molecular biology grade water undergoes advanced purification to achieve 18 MΩ·cm resistivity, including reverse osmosis, deionization, autoclaving, and final membrane filtration. Each lot is supported by comprehensive quality control and Certificate of Analysis (COA) documentation, ensuring full traceability, reproducibility, and compliance with laboratory quality standards.

Validated Nuclease-Free Performance

Each lot is tested for RNase, exonuclease, and nonspecific endonuclease activity using validated methods. This testing supports the use of the water in workflows where maintaining nucleic acid integrity is critical, including sample preparation, storage, and downstream molecular applications.

PCR-Compatible, DEPC-Free Formulation

Unlike DEPC-treated water, this formulation eliminates the risk of residual chemical interference from Diethyl pyrocarbonate. DEPC and its by-products can inhibit DNA polymerase and compromise PCR efficiency. By avoiding DEPC treatment, this water supports reliable performance in PCR, qPCR, dPCR, and next-generation sequencing, while also enabling use in DEPC-sensitive applications such as oocyte microinjection.

Consistent Performance for Critical Workflows

With a pH range of 6 to 8 and molecular biology grade specifications, this nuclease-free water is optimized for consistent performance in critical workflows where contamination control, data reliability, and sample integrity are essential.

Order Info

Shipping Condition: Room temperature

Specifications

• pH: 6 to 8
• Grade: Molecular Biology
• Packaging: Bottle
• Treatment(s): Not DEPC-Treated
• Quantity: 10 x 50 mL
• Purification Method: Autoclaved, Membrane-Filtered
• Shipping Condition: Room Temperature
• Product Line: Ambion

Frequently Asked Questions (FAQs)

What is the expiration date for Nuclease-Free Water (Cat. No. AM9937)?

This will be covered by our standard 12-month warranty as outlined in Section 8.1 of our Terms and Conditions of Sale. The 12-month warranty period begins from the date we ship the product to you. If your warranty has expired, the product may still perform as expected (when stored properly), we are just not able to guarantee or issue free-of-charge replacements in the case of poor performance.

I would like to order a TaqMan SNP Genotyping Assay. Does the kit include everything I need to perform my experiment or do I need to purchase reagents separately?

Each predesigned TaqMan SNP Genotyping Assay is delivered in a single tube consisting of two differentially labeled allele-specific TaqMan MGB (minor groove binding) probes and a pair of PCR primers. For more information, please see the following link: https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-applications/genetic-variation-analysis-using-real-time/snp-genotyping-with-real-time-pcr.html

Besides the TaqMan SNP Genotyping Assay, you would need to purchase a Genotyping Master Mix and nuclease-free water. A list of compatible master mixes can be found in the following link: https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0009593_TaqManSNP_UG.pdf.

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.