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Invitrogen™ pMIR-REPORT™ miRNA Expression Reporter Vector System
Description
The pMIR-REPORT™ miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell measurement of miRNA expression. This validated reporter system contains two mammalian expression vectors. The pMIR-REPORT Luciferase miRNA Expression Reporter Vector contains firefly luciferase under the control of a mammalian promoter/terminator system, with an miRNA target cloning region downstream of the luciferase translation sequence. This vector is optimized for cloning miRNA targets and evaluating miRNA regulation. A second vector, pMIR-REPORT Beta-galactosidase Reporter Control Vector, is provided for normalizing transfection efficiency.
Applications:
pMIR-REPORT Luciferase is designed for cloning and testing putative miRNA binding sites. pMIR-REPORT Luciferase can be transfected into mammalian cells to evaluate endogenous miRNA expression, or used to evaluate the up- or down-regulation resulting from the transfection of Pre-miR™ miRNA Molecules or Anti-miR miRNA Inhibitor Molecules, respectively.
- Contains two mammalian expression vectors: one for cloning miRNA targets and evaluating miRNA regulation, and the other for the normalization of transfection efficiency
- Clone miRNA targets and evaluate miRNA regulation
- Screen putative miRNA target sequences
- Includes both reporter and control vectors
RNAi, RNAi, Epigenetics and Non-Coding RNA Research, Vector-Based RNAi, miRNA and Non-Coding RNA Analysis, miRNA Functional Analysis
Order Info
Shipping Condition: Dry Ice
Specifications
Specifications
| Product Type | RNAi Expression Vector Kit |
| Gene | Beta-Gal (lacZ), Luciferase |
| Content And Storage | pMIR-REPORT™ Luciferase and pMIR-REPORT™ β-gal Control Plasmid are both supplied as glycerol stocks in E. coli and should be stored at –70°C. |
| Cloning Method | Restriction Enzyme ⁄ MCS |
| Constitutive or Inducible System | Constitutive |
| Delivery Type | Transfection |
| RNAi Type | miRNA |
| Selection Agent (Eukaryotic) | Puromycin |
| Shipping Condition | Dry Ice |
| Vector | pMIR |
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For Research Use Only. Not for use in diagnostic procedures.
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