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Invitrogen™ pMIR-REPORT™ miRNA Expression Reporter Vector System

Catalog No. AM5795
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Catalog No. AM5795 Supplier Invitrogen™ Supplier No. AM5795
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Description

For the measurement of miRNA expression in cells

The pMIR-REPORT™ miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell measurement of miRNA expression. This validated reporter system contains two mammalian expression vectors. The pMIR-REPORT Luciferase miRNA Expression Reporter Vector contains firefly luciferase under the control of a mammalian promoter/terminator system, with an miRNA target cloning region downstream of the luciferase translation sequence. This vector is optimized for cloning miRNA targets and evaluating miRNA regulation. A second vector, pMIR-REPORT Beta-galactosidase Reporter Control Vector, is provided for normalizing transfection efficiency.

Applications:
pMIR-REPORT Luciferase is designed for cloning and testing putative miRNA binding sites. pMIR-REPORT Luciferase can be transfected into mammalian cells to evaluate endogenous miRNA expression, or used to evaluate the up- or down-regulation resulting from the transfection of Pre-miR™ miRNA Molecules or Anti-miR miRNA Inhibitor Molecules, respectively.

  • Contains two mammalian expression vectors: one for cloning miRNA targets and evaluating miRNA regulation, and the other for the normalization of transfection efficiency
  • Clone miRNA targets and evaluate miRNA regulation
  • Screen putative miRNA target sequences
  • Includes both reporter and control vectors

RNAi, RNAi, Epigenetics and Non-Coding RNA Research, Vector-Based RNAi, miRNA and Non-Coding RNA Analysis, miRNA Functional Analysis

Order Info

Shipping Condition: Dry Ice

TRUSTED_SUSTAINABILITY

Specifications

Product Type RNAi Expression Vector Kit
Gene Beta-Gal (lacZ), Luciferase
Content And Storage pMIR-REPORT™ Luciferase and pMIR-REPORT™ β-gal Control Plasmid are both supplied as glycerol stocks in E. coli and should be stored at –70°C.
Cloning Method Restriction Enzyme ⁄ MCS
Constitutive or Inducible System Constitutive
Delivery Type Transfection
RNAi Type miRNA
Selection Agent (Eukaryotic) Puromycin
Shipping Condition Dry Ice
Vector pMIR
Promoter CMV
Product Line Ambion, pMIR-REPORT
Quantity 1 kit
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For Research Use Only. Not for use in diagnostic procedures.

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