Invitrogen™ PureLink™ Plant RNA Reagent

Description

Includes

Supplied as a ready-to-use solution.

Plant RNA Reagent is a single-component solution optimized for the isolation of high yields of high-quality total RNA from a variety of plant tissues, especially those rich in polyphenolics (e.g., conifer needles) or starch (e.g., potato tuber or seeds).

Plant RNA Reagent is a single-component solution optimized for the isolation of high yields of high-quality total RNA from a variety of plant tissues, especially those rich in polyphenolics (e.g., conifer needles) or starch (e.g., potato tuber or seeds).

Improved quality and purity of plant RNA
Plant RNA Reagent is optimized to have less sample DNA contamination, to be twice as effective as TRIzol Reagent, and have a simplified RNA extraction protocol for minimized time and labor. The end result is high-yield, high-purity RNA (determined by A₂₆₀/A₂₈₀ and gel analysis) from a variety of plant material sources.

Works for milligram to gram quantities of starting material
Plant RNA Reagent provides greater flexibility for sample size and processing formats, and can be used for both small-scale (up to 0.1 g plant tissue) and large-scale (0.1–5 g plant tissue) total RNA isolation. The amount supplied (100 mL) is sufficient for 200 reactions using 100 mg of tissue, or 4 reactions using 5g of tissue.

• Isolate high-quality RNA from difficult plant materials
• Use for small-scale and large-scale RNA isolations
• Fewer resources used, less hazardous, less waste

Isolated RNA is ideal for a variety of applications and products:

• cDNA library construction kits
• Real-Time PCR (qPCR)
• Northern blotting kits and reagents
• Poly(A) selection kit RNase control reagents and assays

Not high-throughput compatible (Manual)

Specifications

• Content And Storage: PureLink™ Plant RNA Reagent is supplied as a ready-to-use solution. Store at +4°C.
• Isolation Technology: Organic Extraction
• Purification Time: 1 hr.
• Sample Type: Plant Samples
• Final Product Type: Total RNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• For Use With (Application): Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Nuclease Protection Assays, Northern Blotting
• No. of Reactions: 200 Preps
• Quantity: 100 mL
• Scale: Scaleable
• Starting Material Amount: Up to 1 g tissue, Up to 1 x 10^7 cells
• Target: Total RNA

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.