Invitrogen™ PureLink™ Plant RNA Reagent
Total RNA isolation from plant tissues
Manufacturer: Invitrogen™ 12322012
Plant RNA Reagent is a single-component solution optimized for the isolation of high yields of high-quality total RNA from a variety of plant tissues, especially those rich in polyphenolics (e.g., conifer needles) or starch (e.g., potato tuber or seeds).
Improved quality and purity of plant RNA Plant RNA Reagent is optimized to have less sample DNA contamination, to be twice as effective as TRIzol Reagent, and have a simplified RNA extraction protocol for minimized time and labor. The end result is high-yield, high-purity RNA (determined by A260/A280 and gel analysis) from a variety of plant material sources.
Works for milligram to gram quantities of starting material Plant RNA Reagent provides greater flexibility for sample size and processing formats, and can be used for both small-scale (up to 0.1 g plant tissue) and large-scale (0.1–5g plant tissue) total RNA isolation. The amount supplied (100mL) is sufficient for 200 reactions using 100mg of tissue, or 4 reactions using 5g of tissue.
- Isolate high-quality RNA from difficult plant materials
- Use for small-scale and large-scale RNA isolations
- Fewer resources used, less hazardous, less waste
Isolated RNA is ideal for a variety of applications and products:
- cDNA library construction kits
- Real-Time PCR (qPCR)
- Northern blotting kits and reagents
- Poly(A) selection kit RNase control reagents and assays
Not high-throughput compatible (Manual)
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Plant Cells
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|PureLink™ Plant RNA Reagent is supplied as a ready-to-use solution. Store at +4°C.|
|Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Nuclease Protection Assays, Northern Blotting|
|Up to 1 g tissue, Up to 1 x 10^7 cells|
|Not High-throughput Compatible (Manual)|
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For Research Use Only. Not for use in diagnostic procedures.