Invitrogen™ PureLink™ RNA Micro Scale Kit
Provides rapid purification and concentration of total RNA from a variety of samples (e.g., LCM tissues, fine needle aspirates, and FACS-sorted cells)
Manufacturer: Invitrogen™ 12183016
A simple and reliable technology for everyday RNA purification
PureLink™ technology combines guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can be completed in typically less than 15 minutes with >85% recovery from the first elution. The kit can be used for clean-up of purified RNA from enzymatic reactions, desalting of RNA samples after phenol extraction or ethanol precipitation, and concentration of RNA. Purify RNA from up to 10mg of tissue or 105 cells. Clean up and concentrate RNA amounts ranging from pg to 100μg in volumes as small as 10μL. Purified RNA is ready for use in downstream applications such as RT-PCR, qRT-PCR, northern blots, cDNA library construction, and nuclease protection assays.
A simple and rapid procedure
Homogenize your sample in lysis buffer, add 70% ethanol, and mix. Deposit the resulting solution into a PureLink Micro kit column with collection tube, and centrifuge for one minute. Nucleic acids bind to the PureLink silica membrane in the column, and after subsequent washes and on-column digestion with DNase, purified RNA can be eluted with RNase-free water.
- Effective: Expect excellent RNA recovery (up to 100μg total RNA) from small samples
- Convenient and safe: The extraction procedure uses no phenol/chloroform, CsCl gradients, or precipitation with either LiCl or ethanol
- Fast: Obtain high-quality total RNA, typically in just 15 minutes
- Reliable: Obtain ready-to-use RNA suitable for any downstream application
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|Box 1 (PureLink™ RNA Micro Kit):
• Lysis Buffer (125 ml)
• Wash Buffer I (50 ml)
• Wash Buffer II (15 ml)
• RNase-Free Water (15.5 ml)
• PureLink™ Micro Kit Columns with collection tubes (50 each)
• Collection Tubes (50 each)
• Recovery Tubes (50 each)
Box 2 (PureLink™ DNase/Carrier RNA)
• Lyophilized PureLink™ DNase (1500 Units)
• PureLink™ On-Column DNase 2X Buffer (1 ml)
• RNase-Free Water (1 ml)
• Lyophilized PureLink™ Carrier RNA (300 µg)
Store Box 2 at 4°C. Store remaining components at room temperature.
|Silica Spin Column|
|10 µL, 5 µL 20 µl|
|Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Nucleic Acid Labeling, Northern Blotting, cDNA Library Construction|
|Less than 10 mgs tissue, Up to 5 x 10^5 cells|
|Bacteria, Fine Needle Aspirates (FNA), Laser Capture Microdissection (LCM), Mammalian Cells, Plant, Tissue (Mammalian), Yeast|
|Not High-throughput Compatible (Manual)|
|Molecular Biology Grade|
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For Research Use Only. Not for use in diagnostic procedures.