Invitrogen™ PureLink™ Total RNA Blood Kit

Description

PureLink Total RNA Blood Kit allows purification of high-quality total RNA from 50 to 500 μL fresh, whole mammalian blood, using non-phenol based purification in a simple spin column based protocol.

PureLink Total RNA Blood Kit allows purification of high-quality total RNA from 50 to 500 μL fresh, whole mammalian blood, using non-phenol based purification in a simple spin column based protocol.

Simple RNA Purification from Whole Blood
PureLink Total RNA Blood Kit is designed for purifying from 50 to 500 μL fresh whole blood or approximately 3.5 x 10⁶ leukocytes (white blood cells) in less than 1 hour. The entire protocol can be done in spin tubes and spin columns.

Leukocyte Enrichment for Improved RNA Purification
Whole blood consists of non-nucleated erythrocytes (red blood cells, 4 - 6 million/mm³) and leukocytes, with the latter present in the minority (5000 - 7000/mm³). Purifying total RNA from whole blood without the removal of erythrocytes results in low RNA yields and clogging of purification columns. The distinct feature of the PureLink Total RNA Blood Kit is that it employs a leukocyte enrichment step. Hypotonic shock is used to selectively lyse erythrocytes while not affecting leukocytes. Non-nucleated erythrocytes are efficiently depleted, thus significantly reducing cell debris that may otherwise interfere with RNA purification and other downstream applications. Consequently, yields of high-quality total RNA are obtained using the PureLink Total RNA Blood Kit.

• RNA purification from whole blood in under an hour
• Yields high-quality RNA devoid of non-nucleated erythrocytes

Purified RNA is Suitable for Use with a Variety of Downstream Applications and Products:

• Northern Blotting Kits & Reagents
• Nuclease protection assays
• Microarray
• cDNA synthesis
• Real-Time PCR (qPCR)

Not high-throughput compatible (Manual)

Applications
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Blood

Order Info

Shipping Condition: Room temperature

Specifications

• Content And Storage: • 17.5 mL Lysis Buffer L3; store at room temperature
  • 175 mL Lysis Buffer L5; room temperature
  • 60 mL Wash Buffer W4; room temperature
  • 10 mL Wash Buffer W5; room temperature
  • 5 mL RNase-free Water; room temperature
  • 50 Spin Cartridges with Collection Tubes; room temperature
  • 50 Wash Tubes (2 mL); room temperature
  • 50 Elution Tubes (1.7 mL); room temperature
• Isolation Technology: Silica Spin Column
• Purification Time: 20 min. (After sample homogenization)
• Elution Volume: 30–100 μL
• Sample Type: Blood
• Final Product Type: Total RNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
• Quantity: 50 preps
• Scale: Mini
• Shipping Condition: Room Temperature
• Starting Material Amount: Blood: ≤500 μL
• Yield: 1000 μg (Binding capacity)

For Research Use Only. Not for use in diagnostic procedures.