Invitrogen™ PureLink™ Total RNA Blood Kit

Catalog No.	Quantity
K156001	50 preps

Catalog No. K156001 Supplier Invitrogen™ Supplier No. K156001

Description

PureLink Total RNA Blood Kit allows purification of high-quality total RNA from 50 to 500 μL fresh, whole mammalian blood, using non-phenol based purification in a simple spin column based protocol.

PureLink Total RNA Blood Kit allows purification of high-quality total RNA from 50 to 500 μL fresh, whole mammalian blood, using non-phenol based purification in a simple spin column based protocol.

Simple RNA Purification from Whole Blood
PureLink Total RNA Blood Kit is designed for purifying from 50 to 500 μL fresh whole blood or approximately 3.5 x 10⁶ leukocytes (white blood cells) in less than 1 hour. The entire protocol can be done in spin tubes and spin columns.

Leukocyte Enrichment for Improved RNA Purification
Whole blood consists of non-nucleated erythrocytes (red blood cells, 4 - 6 million/mm³) and leukocytes, with the latter present in the minority (5000 - 7000/mm³). Purifying total RNA from whole blood without the removal of erythrocytes results in low RNA yields and clogging of purification columns. The distinct feature of the PureLink Total RNA Blood Kit is that it employs a leukocyte enrichment step. Hypotonic shock is used to selectively lyse erythrocytes while not affecting leukocytes. Non-nucleated erythrocytes are efficiently depleted, thus significantly reducing cell debris that may otherwise interfere with RNA purification and other downstream applications. Consequently, yields of high-quality total RNA are obtained using the PureLink Total RNA Blood Kit.

• RNA purification from whole blood in under an hour
• Yields high-quality RNA devoid of non-nucleated erythrocytes

Purified RNA is Suitable for Use with a Variety of Downstream Applications and Products:

• Northern Blotting Kits & Reagents
• Nuclease protection assays
• Microarray
• cDNA synthesis
• Real-Time PCR (qPCR)

Not high-throughput compatible (Manual)

Applications
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Blood

Order Info

Shipping Condition: Room temperature

Specifications

• Content And Storage: • 17.5 mL Lysis Buffer L3; store at room temperature
  • 175 mL Lysis Buffer L5; room temperature
  • 60 mL Wash Buffer W4; room temperature
  • 10 mL Wash Buffer W5; room temperature
  • 5 mL RNase-free Water; room temperature
  • 50 Spin Cartridges with Collection Tubes; room temperature
  • 50 Wash Tubes (2 mL); room temperature
  • 50 Elution Tubes (1.7 mL); room temperature
• Isolation Technology: Silica Spin Column
• Purification Time: 20 min. (After sample homogenization)
• Elution Volume: 30–100 μL
• Sample Type: Blood
• Final Product Type: Total RNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
• Quantity: 50 preps
• Scale: Mini
• Shipping Condition: Room Temperature
• Starting Material Amount: Blood: ≤500 μL
• Yield: 1000 μg (Binding capacity)

Frequently Asked Questions (FAQs)

Do you have any tips for RNA isolation when working with blood samples?

Please visit our website for tips for working with blood samples.

What is the level of detection for somatic variants with the Oncomine Myeloid Research Assay GX?

Detection of somatic variants with the Oncomine Myeloid Research Assay GX has been verified down to 5% allele frequency.

Which extraction kits are compatible with Oncomine Myeloid Research Assay GX?

Here is a list of kits that are compatible with Oncomine Myeloid Research Assay GX:

Nucleic acid isolation - RNA samples
- MagMAX mirVana Total RNA Isolation Kit, Cat. No. A27828
- PureLink Total RNA Blood Kit, Cat. No. K156001
- RNaseZap RNase Decontamination Solution, Cat. No. AM9780, AM9782, AM9784

Nucleic acid isolation - DNA samples
- MagMAX DNA Multi-Sample Ultra 2.0 Kit, Cat. No. A36570
- PureLink Genomic DNA Mini Kit, Cat. No. K1820-01

What are the sample input requirements for Oncomine Myeloid Research Assay GX?

The recommended minimum sample input is 27.75 ng of DNA (1.11 ng/µL minimum concentration) and 14.25 ng of RNA (0.95 ng/µL minimum concentration).

What sample controls should I use with the Oncomine Myeloid Research Assay GX?

The recommended controls are:

DNA Controls:
- Seraseq® Myeloid Mutation DNA Mix, Cat. No. 0710-0408 (SeraCare®)
- Myeloid DNA Reference Standard, Cat. No. HD829 (Horizon™)
- AcroMetrix™ Oncology Hotspot Control, Cat. No. 969056

RNA Controls:
- Universal Human Reference RNA, Cat. No. 740000 (Agilent™)
- Seraseq® Myeloid Fusion RNA Mix, Cat. No. 0710-0407 (SeraCare®)

How many Oncomine Myeloid Research Assay GX samples can be multiplexed on a GX5 Chip?

Multiplex sequencing of up to 8 Oncomine Myeloid Research Assay GX samples (8 DNA+ 8 RNA) per lane on a GX5 Chip can be performed in a single run. There are 4 lanes on the GX5 Chip, so 32 total samples (32 DNA+ 32 RNA) can be run on one GX5 Chip (8 samples per run).

What are the components of Oncomine Myeloid Research Assay GX?

The Oncomine Myeloid Research Assay GX (Cat. No. A47857) includes the 2-pool DNA panel, the 1-pool RNA panel, Genexus Strip 1, and Genexus Strip 2-AS. The DNA panel is provided in two packs of 8 tubes (4 tubes of Myeloid DNA Pool 1 and 4 tubes of Myeloid DNA Pool 2 per pack). The RNA panel is provided in one pack of 8 tubes (Myeloid RNA Pool 1). Each primer pool in the panel is provided in pairs of tubes, where each tube pair contains one tube with primers in position 1 and one empty uncapped tube in position 2. Three 8-strip packs of the Genexus Strip 1 and Genexus Strip 2-AS (24 total of each strip) are provided with each kit.

How many samples can I run using Oncomine Myeloid Research Assay GX?

The contents of each Oncomine Myeloid Research Assay GX kit are sufficient for up to 32 samples (32 x 3‑pool reactions: 32 x 2‑pool DNA reactions and 32 x 1‑pool RNA reactions).

How many primer pools are present in the Oncomine Myeloid Research Assay GX?

The Oncomine Myeloid Research Assay GX includes 3 pools of Ion AmpliSeq oligonucleotide primers (a 2-pool DNA panel and a 1-pool RNA panel).

What is the Oncomine Myeloid Research Assay GX gene content?

The Oncomine Myeloid Research Assay GX targets key genes and fusions associated with major myeloid disorders, including acute myeloid leukemia (AML), myeloid dysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myeloid leukemia (CML), chronic myelomonocytic leukemia (CMML), and juvenile myelomonocytic leukemia (JMML). The DNA panel comprises 40 key genes while the RNA panel includes a broad fusion panel of 29 driver genes, enabling detection over 600 unique fusion isotypes. See flyer for gene targets (https://assets.thermofisher.com/TFS-Assets/CSD/Flyers/Oncomine_myeloid_assay_gx_flyer.pdf).

What is the Oncomine Myeloid Research Assay GX designed for ?

The Oncomine Myeloid Research Assay GX is a comprehensive targeted next-generation sequencing (NGS) assay designed for sensitive detection of myeloid disorder-associated DNA mutations and fusion transcripts in blood and bone marrow samples.

For Research Use Only. Not for use in diagnostic procedures.