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Invitrogen™ Retic Lysate IVT™ Kit Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Efficiently translates high molecular weight proteins and both capped and uncapped mRNAs

$278.00 - $293.00

Specifications

For Use With (Application) In Vitro Transcription
Quantity 60 reactions
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Includes: Retic Lysate should be stored at -80°C. 20X Translation Mix minus-Met, 20X Translation Mix minus-Leu, High Salt Mix minus-Met, Low Salt Mix minus-Met, and Capped Xef-1 RNA should be stored below -70°C. Nuclease-free Water can be stored at any temperature.

Products 2
Catalog Number Mfr. No. Quantity Product Type Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Product Type Price Quantity & Availability  
AM1200 Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.
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Invitrogen™
AM1200
60 reactions Kit
Each for $293.00
Add to cart
 
AM1200M Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.
View Documents
Invitrogen™
AM1200M
60 reactions Kit with manual
Each for $278.00
Add to cart
 
Description

Description

The Retic Lysate IVT™ Kit efficiently translates in vitro-synthesized transcripts, poly(A) RNA, and total RNA, including difficult-to-translate RNAs. The optimized lysate results in high protein yield, biological activity, and consistent performance. A report in Nucleic Acids Research (1) found that some commercial reticulocyte lysates had poor fidelity for initiation at the appropriate AUG initiator codon. The Retic Lysate IVT Kit was tested using Kozak's constructs and it demonstrated high fidelity for initiating at the proper AUG.

  • Efficiently translates a wide variety of high molecular weight proteins and both capped and uncapped mRNAs
  • Accommodates large volume of template mRNA
How it works
The lysate has been optimized for translation by the addition of an ATP-regenerating system, hemin, and calf liver tRNA. Included in the kit is an optimized Translation Mix (-Met or -Leu) for the translation of both capped and uncapped messages along with High and Low Salt buffers. These buffers can be used to maximize protein yield and activity from difficult-to-translate sequences.

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Specifications

Specifications

In Vitro Transcription
60 reactions
Safety and Handling

Safety and Handling

SDS

For Research Use Only. Not for use in diagnostic procedures.

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