Invitrogen RiboPure RNA Purification Kit, yeast
Enzymatic pretreatment not required
Manufacturer: Invitrogen AM1926
For the isolation of extremely high-quality RNA from yeast without the use of enzymatic pretreatments that may alter gene expression profiles. Each Invitrogen kit includes sufficient reagents for 50 purifications each from 107 to 108 yeast cells.
It has traditionally been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Many protocols use enzymatic pretreatments that may affect gene expression patterns. The RiboPure-Yeast RNA Isolation Kit uses no enzymatic pretreatments and thus does not alter expression profiles. It combines a simple and efficient glass bead/one step phenolmediated disruption using Invitrogen's Vortex Bead Beater Adapter followed by a glass fiber filter based RNA purification step. After DNase treatment with the included DNA-free Reagents, the resulting RNA is exceptionally pure containing little to no genomic DNA (less than 0.05%). The kit has been used extensively at Invitrogen to isolate high-quality RNA from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris, and Candida albicans. The kit provides the highest yield of any method tested from each of these species.
- Highest yields of total RNA available
- No enzymatic pretreatment required
- Includes DNA-free Reagents
Not high-throughput compatible (Manual)
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Yeast
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|Total RNA (Yeast)|
|Organic Extraction, Spin Column|
|Not High-throughput Compatible (Manual)|
|Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting, cDNA Library Construction|
|Store 1.5 mL Screw Cap Tubes, Filter Cartridges and Collection Tubes at room temperature. Store Lysis Buffer, 10% SDS, Phenol:Chloroform:IAA, Binding Buffer, Wash Solution 1, Wash Solution 2⁄3 Concentrate and Elution Buffer at 4°C. Store Zirconia Beads, 10X DNase I Buffer, DNase 1 and DNase Inactivation Reagent at -20°C.|
|Up to 10^8 cells|
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For Research Use Only. Not for use in diagnostic procedures.