Invitrogen™ RiboPure™ RNA Purification Kit, yeast  

Enzymatic pretreatment not required

Manufacturer:  Invitrogen™ AM1926

Catalog No. AM1926

Description

For the isolation of extremely high-quality RNA from yeast without the use of enzymatic pretreatments that may alter gene expression profiles. Each Invitrogen kit includes sufficient reagents for 50 purifications each from 10⁷ to 10⁸ yeast cells.
It has traditionally been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Many protocols use enzymatic pretreatments that may affect gene expression patterns. The RiboPure-Yeast RNA Isolation Kit uses no enzymatic pretreatments and thus does not alter expression profiles. It combines a simple and efficient glass bead/one step phenol–mediated disruption using Invitrogen's Vortex Bead Beater Adapter followed by a glass fiber filter– based RNA purification step. After DNase treatment with the included DNA-free™ Reagents, the resulting RNA is exceptionally pure containing little to no genomic DNA (less than 0.05%). The kit has been used extensively at Invitrogen to isolate high-quality RNA from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris, and Candida albicans. The kit provides the highest yield of any method tested from each of these species.

• Highest yields of total RNA available
• No enzymatic pretreatment required
• Includes DNA-free™ Reagents

Not high-throughput compatible (Manual)

Applications:

DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Yeast

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Specifications

• Final Product Type: Total RNA (Yeast)
• Purification Target: Total RNA
• Isolation Technology: Organic Extraction, Spin Column
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Product Line: Ambion™, RiboPure™

• For Use With (Application): Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting, cDNA Library Construction
• Sample Type: Yeast
• Content And Storage: Store 1.5 mL Screw Cap Tubes, Filter Cartridges and Collection Tubes at room temperature. Store Lysis Buffer, 10% SDS, Phenol:Chloroform:IAA, Binding Buffer, Wash Solution 1, Wash Solution 2⁄3 Concentrate and Elution Buffer at 4°C. Store Zirconia Beads, 10X DNase I Buffer, DNase 1 and DNase Inactivation Reagent at -20°C.
• Starting Material Amount: Up to 10^8 cells

For Research Use Only. Not for use in diagnostic procedures.