Learn More
Invitrogen™ RNAqueous™-4PCR Total RNA Isolation Kit

Description
Includes
600μL 10X DNase I Buffer; 45μL DNase I 2U/μL; 1mL Formaldehyde Load Dye; 100μL Linear Acrylamide 5mg/mL; 50μL Positive Control Primers; 1mL 5M Ammonium Acetate; 35mL Water for 64% Ethanol; 600mL DNase Inactivation Reagent; 30mL Lysis/Binding Solution; 25mL Wash Solution 1; 35mL Wash Solution 2/3; 3 Pestles for tissue disruption; 30 Filter Cartridges; 60 Collection Tubes; 25mL Elution Solution
The RNAqueous-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 to 75 mg) or from cells (∽100 cells to ∽1 x 107 cells), using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR.
- DNA-free RNA for RT-PCR
- Rapid, phenol-free total RNA isolation
- Novel reagent for rapid and safe removal of DNase and divalent cations
Using the RNAqueous-4PCR Kit
The RNAqueous-4PCR Kit is designed for isolatation of RNA for applications such as RT-PCR, that would benefit from its robust genomic DNA removal capabilities. When used in conjunction with RNAlater™ Tissue Collection: RNA Stabilization Solution, most tissue samples can be stored at 4°C and then easily disrupted in standard microfuge tubes using the pestles provided in the kit. No need for snap-freezing in liquid nitrogen or grinding in a mortar and pestle.
Not high-throughput compatible (Manual)
Applications:
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues

Specifications
Specifications
Content And Storage | • 600 μL 10X DNase 1 Buffer; store at -20°C • 45 μL DNase 1; -20°C • 1 mL Formaldehye Load Dye; -20°C • 100 μL Linear Acrylamide; -20°C • 50 μL Positive Control Primers; -20°C • 1 mL 5M Ammonium Acetate; -20°C • 35 mL Water; 4°C • 600 μL DNase Inactivation Reagent; 4°C • 30 mL Lysis/Binding Solution; 4°C • 25 mL Wash Solution #1; 4°C • 35 mL Wash Solution 2/3 (concentrated); 4°C • 3 Pestles; room temperature • 30 Filter Cartridges; room temperature • 60 Collection Tubes; room temperature • 25 mL Elution Solution; room temperature |
Isolation Technology | Silica Spin Column |
Purification Time | 10 min. (After sample homogenization) |
Elution Volume | 50–120 μL |
Sample Type | Tissue, Cells |
Final Product Type | Total RNA |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
For Use With (Application) | RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling |
Quantity | 30 Preps |
Shipping Condition | Box 1: Dry Ice, Box 2: Room Temperature |
Show More |
For Research Use Only. Not for use in diagnostic procedures.
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.