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Invitrogen™ RNAqueous™-4PCR Total RNA Isolation Kit

Catalog No. AM1914
Encompass_Preferred
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Quantity:
30 Preps

Includes

600μL 10X DNase I Buffer; 45μL DNase I 2U/μL; 1mL Formaldehyde Load Dye; 100μL Linear Acrylamide 5mg/mL; 50μL Positive Control Primers; 1mL 5M Ammonium Acetate; 35mL Water for 64% Ethanol; 600mL DNase Inactivation Reagent; 30mL Lysis/Binding Solution; 25mL Wash Solution 1; 35mL Wash Solution 2/3; 3 Pestles for tissue disruption; 30 Filter Cartridges; 60 Collection Tubes; 25mL Elution Solution

The RNAqueous-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 to 75 mg) or from cells (∽100 cells to ∽1 x 107 cells), using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

The RNAqueous-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 to 75 mg) or from cells (∽100 cells to ∽1 x 107 cells), using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR.

  • DNA-free RNA for RT-PCR
  • Rapid, phenol-free total RNA isolation
  • Novel reagent for rapid and safe removal of DNase and divalent cations

Using the RNAqueous-4PCR Kit

The RNAqueous-4PCR Kit is designed for isolatation of RNA for applications such as RT-PCR, that would benefit from its robust genomic DNA removal capabilities. When used in conjunction with RNAlater™ Tissue Collection: RNA Stabilization Solution, most tissue samples can be stored at 4°C and then easily disrupted in standard microfuge tubes using the pestles provided in the kit. No need for snap-freezing in liquid nitrogen or grinding in a mortar and pestle.

Not high-throughput compatible (Manual)

Applications:

DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage • 600 μL 10X DNase 1 Buffer; store at -20°C
• 45 μL DNase 1; -20°C
• 1 mL Formaldehye Load Dye; -20°C
• 100 μL Linear Acrylamide; -20°C
• 50 μL Positive Control Primers; -20°C
• 1 mL 5M Ammonium Acetate; -20°C
• 35 mL Water; 4°C
• 600 μL DNase Inactivation Reagent; 4°C
• 30 mL Lysis/Binding Solution; 4°C
• 25 mL Wash Solution #1; 4°C
• 35 mL Wash Solution 2/3 (concentrated); 4°C
• 3 Pestles; room temperature
• 30 Filter Cartridges; room temperature
• 60 Collection Tubes; room temperature
• 25 mL Elution Solution; room temperature
Isolation Technology Silica Spin Column
Purification Time 10 min. (After sample homogenization)
Elution Volume 50–120 μL
Sample Type Tissue, Cells
Final Product Type Total RNA
High-throughput Compatibility Not High-throughput Compatible (Manual)
For Use With (Application) RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity 30 Preps
Shipping Condition Box 1: Dry Ice, Box 2: Room Temperature
Starting Material Amount Tissue: ≤75 mg, Cells: ≤107
Yield ≤10 μg per 1 mg tissue, ≤1 μg per 1 x 105 cells
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For Research Use Only. Not for use in diagnostic procedures.

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