Invitrogen™ RNAqueous™-96 Automated Kit

Catalog No.	Quantity
AM1812	384 Preps

Catalog No. AM1812 Supplier Invitrogen™ Supplier No. AM1812

Description

Includes

4 Filter Plates, 4 Collection Plate with lids, 4 Culture Plates, 1 Heat Plate with lid, 100mL Nuclease-free Water, 100mL Elution Solution, 100mL Lysis/Binding Solution, 100mL Rebinding Mix, and 2 x 175mL Wash Solution Concentrate, (1.25mL DNase I (20U/μL) and 12mL DNase 1 Buffer

The Invitrogen RNAqueous-96 Automated Kit is used for automated, phenol-free total RNA isolation from 100-500,000 cells or 0.1–1 mg tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

The Invitrogen RNAqueous-96 Automated Kit is used for automated, phenol-free total RNA isolation from 100-500,000 cells or 0.1–1 mg tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The kit contains enough reagents for 384 (4 x 96) RNA purifications. The RNAqueous-96 Automated Kit uses an RNA-binding glass-fiber filter to provide high, reproducible yields of intact RNA. An optional on-the-filter DNase treatment can be performed to ensure removal of genomic DNA for RT-PCR applications.

Specifications

• Content And Storage: • 4 Filter Plates; store at room temperature
  • 4 Collection Plates with lids; room temperature
  • 4 Culture Plates; room temperature
  • 1 Heat Plate with Lid; room temperature
  • 100 mL Nuclease-free Water; room temperature
  • 100 mL Elution Solution; room temperature
  • 100 mL Lysis/Binding Solution; 4°C
  • 100 mL Rebinding Mix; 4°C
  • 2 x 175 mL Wash Solution (concentrated); 4°C
  • 1.25 mL DNase 1; -20°C
  • 12 mL DNase 1 Buffer; -20°C
• Isolation Technology: Vacuum Filter Plate (Glass Fiber Filter)
• Elution Volume: 105 μL
• Sample Type: Cells, Tissue
• Final Product Type: Total RNA
• High-throughput Compatibility: High-throughput Compatible
• For Use With (Equipment): MultiPROBE™ II HT, Biomek™ 2000, Liquid Handling Systems
• For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
• Quantity: 384 Preps
• Shipping Condition: Box 1: Dry Ice
  Box 2: Room Temperature
• Starting Material Amount: Cells: ≤10⁵
  Tissue: 0.1–1 mg

Frequently Asked Questions (FAQs)

What are the differences in the RNAqueous kits you offer?

RNaqueous kits are based on a rapid, filter-based RNA isolation system that does not require phenol, chloroform, or other toxic organic chemicals. The RNAqueous Total RNA Isolation Kit is designed for sample sizes of 10-75 mg of tissue or 10^6 to 10^7 cells, whereas the RNAqueous-Midi Total RNA Isolation Kit is designed for tissue samples of up to 0.5 g or 10^9 cells. The RNAqueous-Micro Total RNA Isolation Kit is optimized for the purification of total RNA from micro-sized samples such as 10-500,000 cultured cells, as few as 10 microdissected cells, or up to 10 mg tissue. The RNAqueous-96 Total RNA Isolation Kit utilizes a 96-well plate format for high-throughput RNA isolation from 100 to 2 x 10^6 cells or 0.1 to 1.5 mg of tissue. The RNAqueous-4PCR Total RNA Isolation Kit is designed for RNA isolation from small tissue samples (0.5-75 mg) and small- to medium-sized cultured cell samples (approximately 100-10^7 cells).

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.