Invitrogen™ RNAqueous™-Micro Total RNA Isolation Kit

The RNAqueous™-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.g., from less than 500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤10 mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

Supplier:  Invitrogen™ AM1931

Catalog No. AM1931

Includes: 50 Micro Filter Cartridge Assembly; 50 Micro Elution Tubes; 15mL Wash Solution 1 Concentrate; 28mL Wash Solution 2/3 concentrate; 25 Lysis Solution; 200μL LCM Additive; 55μL DNase 2U/μL; 600μL 10X DNase I Buffer; 150μL DNase Inactivation Reagent; 5mL Elution Solution

Description

The RNAqueous™-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.g., from less than 500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤10 mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. This kit provides:

• Highly concentrated RNA—for increased sensitivity in downstream assays
• Isolation of high-quality RNA from micro-scale samples
• Reagents/protocol for RNA recovery from LCM samples

How it works
Samples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples. A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs. The RNA is eluted in a minimal volume of 20 μL. Residual genomic DNA is efficiently removed using the DNA-free™ system included in the kit. The RNAqueous™-Micro Kit contains enough reagents for 50 RNA isolations of DNA-free RNA.

Not high-throughput compatible (Manual)

Applications
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues

Specifications

• Content And Storage: • 50 Filter Cartridges with Collection Tubes; store at room temperature
  • 50 Elution Tubes (1.5 mL); room temperature
  • 15 mL Wash Solution 1 (concentrated); room temperature
  • 28 mL Wash Solution 2/3 (concentrated); 4°C
  • 25 mL Lysis Solution; 4°C
  • 200 μL LCM Additive; -20°C
  • 55 μL DNase; -20°C
  • 600 μL 10X DNase I Buffer; -20°C
  • 150 μL DNase Inactivation Reagent; -20°C
  • 5 mL Elution Solution; room temperature
• Purification Time: 15 min. (After sample homogenization)
• Sample Type: Cells, Tissue, Laser Capture Microdissection (LCM)
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 50 Preps
• Starting Material Amount: Cells: ≤10⁵
  Tissue: ≤10 mg

• Isolation Technology: Silica Spin Column
• Elution Volume: 10–20 μL
• Final Product Type: Total RNA
• For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
• Shipping Condition: Box 1: Dry Ice
  Box 2: Room Temperature

For Research Use Only. Not for use in diagnostic procedures.