Invitrogen RNAqueous-Micro Total RNA Isolation Kit
Isolates total RNA from micro-scale samples
Manufacturer: Invitrogen AM1931
The RNAqueous-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.g., from <500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤ 10mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR.
- Highly concentrated RNAfor increased sensitivity in downstream assays
- Isolation of high-quality RNA from micro-scale samples
- Reagents/protocol for RNA recovery from LCM samples
Samples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples. A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs. The RNA is eluted in a minimal volume of 20µL. Residual genomic DNA is efficiently removed using the DNA-free system included in the kit. The RNAqueous-Micro Kit contains enough reagents for 50 RNA isolations of DNA-free RNA.
Not high-throughput compatible (Manual)
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues
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|Silica Spin Column|
|Laser Capture Microdissection (LCM), Reverse Transcriptase PCR (RT-PCR), Northern Blotting|
|Cell Cultures, Laser Capture Microdissection (LCM), Cultured Cells|
|• 50 Micro Filter Cartridge Assembly (room temperature)
• 50 Micro Elution Tubes (room temperature)
• 15 ml Wash Solution 1 Concentrate (room temperature)
• 28 ml Wash Solution 2/3 concentrate (4°C)
• 25 Lysis Solution (4 °C)
• 200 µl LCM Additive (-20°C)
• 55 µl DNase 2 U/µl (-20°C)
• 600 µl 10X DNase I Buffer (-20°C)
• 150 µl DNase Inactivation Reagent (-20°C)
• 5 ml Elution Solution (-20°C, 4°C, room temperature)
|Not High-throughput Compatible (Manual)|
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For Research Use Only. Not for use in diagnostic procedures.