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Invitrogen™ RNAqueous™-Micro Total RNA Isolation Kit Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Isolates total RNA from micro-scale samples

Manufacturer:  Invitrogen™ AM1931

Catalog No. AM1931


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Includes: 50 Micro Filter Cartridge Assembly; 50 Micro Elution Tubes; 15mL Wash Solution 1 Concentrate; 28mL Wash Solution 2/3 concentrate; 25 Lysis Solution; 200µL LCM Additive; 55µL DNase 2U/µL; 600µL 10X DNase I Buffer; 150µL DNase Inactivation Reagent; 5mL Elution Solution

Description

Description

The RNAqueous-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.g., from <500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤ 10mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR.

  • Highly concentrated RNA—for increased sensitivity in downstream assays
  • Isolation of high-quality RNA from micro-scale samples
  • Reagents/protocol for RNA recovery from LCM samples
How it works
Samples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples. A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs. The RNA is eluted in a minimal volume of 20µL. Residual genomic DNA is efficiently removed using the DNA-free™ system included in the kit. The RNAqueous-Micro Kit contains enough reagents for 50 RNA isolations of DNA-free RNA.

Not high-throughput compatible (Manual)

Applications:

DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues

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Specifications

Specifications

Total RNA
Total RNA
Silica Spin Column
20 µl
Micro
Laser Capture Microdissection (LCM), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Cell Cultures, Laser Capture Microdissection (LCM), Cultured Cells
• 50 Micro Filter Cartridge Assembly (room temperature)
• 50 Micro Elution Tubes (room temperature)
• 15 ml Wash Solution 1 Concentrate (room temperature)
• 28 ml Wash Solution 2/3 concentrate (4°C)
• 25 Lysis Solution (4 °C)
• 200 µl LCM Additive (-20°C)
• 55 µl DNase 2 U/µl (-20°C)
• 600 µl 10X DNase I Buffer (-20°C)
• 150 µl DNase Inactivation Reagent (-20°C)
• 5 ml Elution Solution (-20°C, 4°C, room temperature)
Not High-throughput Compatible (Manual)
Ambion™, RNAqueous®
SDS
Documents
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For Research Use Only. Not for use in diagnostic procedures.