Invitrogen RNAqueous Total RNA Isolation Kit
Uses glass fiber filter separation technology
Manufacturer: Invitrogen AM1912
The RNAqueous Kit is for phenol-free total RNA isolation from 0.1g to 0.5g of tissue or from 107 to 108 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.
- Convenient syringe-based filtration
- Compatibility with vacuum manifolds when processing multiple samples
The RNAqueous Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
Not high-throughput compatible (Manual)
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues
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|6mL Formaldehyde Load Dye; 60mL Lysis/Binding Solution; 60mL Water for 64% Ethanol; 4mL Lithium Chloride Precipitation Solution; 40mL Wash Solution 1; 80mL Wash Solution 2/3 Concentrate; 50 Filter Cartridges; 100 Collection Tubes; 10mL Elution Solution|
|Not High-throughput Compatible (Manual)|
|Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting, cDNA Library Construction|
|Plant Samples, Viral Samples, Tissue, Yeast, Bacteria, Cells|
|Spin Column (Glass Fiber Filter)|
|• 6 ml Formaldehyde Load Dye (-20°C)
• 60 ml Lysis/Binding Solution (4°C)
• 60 ml Water for 64% Ethanol (4°C)
• 4 ml Lithium Chloride Precipitation Solution (4°C)
• 40 ml Wash Solution 1 (4°C)
• 80 ml Wash Solution 2/3 Concentrate (4°C)
• 50 Filter Cartridges (room temperature)
• 100 Collection Tubes (room temperature)
• 10 ml Elution Solution (-20°C, 4°C, or room temperature)
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