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Invitrogen™ RNAqueous™ Total RNA Isolation Kit

Uses glass fiber filter separation technology

Supplier:  Invitrogen™ AM1912


Catalog No. AM1912

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The RNAqueous Kit is for phenol-free total RNA isolation from 0.1g to 0.5g of tissue or from 107 to 108 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

  • Convenient syringe-based filtration
  • Compatibility with vacuum manifolds when processing multiple samples

How it works
The RNAqueous Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.

Not high-throughput compatible (Manual)


DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues



• 6 mL Formaldehyde Load Dye; store at -20°C
• 60 mL Lysis/Binding Solution; 4°C
• 60 mL Water; 4°C
• 4 mL Lithium Chloride Precipitation Solution; 4°C
• 40 mL Wash Solution #1; 4°C
• 80 mL Wash Solution #2/3 (concentrated); 4°C
• 50 Filter Cartridges; room temperature
• 100 Collection Tubes; room temperature
• 10 mL Elution Solution; room temperature
50–120 μL
Total RNA
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Bacteria: ≤109 cells
Cells: ≤107
Plant: ≤75 mg
Yeast: ≤108 cells
Virus: varies
Enzymatic Reactions: varies
Spin Column (Glass Fiber Filter)
Bacteria, Cells, Plant, Tissue, Yeast, Viruses, Enzymatic Reactions
Not High-throughput Compatible (Manual)
Room Temperature


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