Invitrogen™ Ambion™ T4 RNA Ligase, cloned, 5 U/μL

Description

Catalyzes formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminated nucleic acid acceptor

• Substrates include RNA, DNA, and oligonucleotides. One tube containing 500U (5U/μL) is provided along with 10X Reaction Buffer
• The enzyme can be used for tagging the 5′ ends of mRNA with oligonucleotides for mapping studies (RACE), 3′-end labeling RNA molecules, and circularizing RNA and DNA molecules
• Unit Definition: One unit catalyzes the formation of 1nmol of [5′-³²P]-rA_12-18 into a phosphatase-resistant form in 30 minutes at 37°C

DNA & RNA Purification & Analysis, Nucleic Acid Labeling & Oligo Synthesis, Oligo Labeling, RNA Labeling

Specifications

• Concentration: 5 U/μL
• Content And Storage: T4 RNA Ligase and 10X Reaction Buffer should be stored at –20°C.
• Format: Tube
• Enzyme: Ligase
• Fidelity (vs. Taq): 10 X
• No. of Reactions: 250
• Polymerase: SP6 RNA Polymerase
• Product Line: Ambion™
• Product Type: T4 Rna Ligase
• Quantity: 2,500 units, 5 μL
• Shipping Condition: Dry Ice
• Source: RNA
• Form: Solution

For Research Use Only. Not for use in diagnostic procedures.