Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ Tempus™ Spin RNA Isolation Kit

Catalog No. 4380204
Encompass_Preferred
Click to view available options
Quantity:
50 Preps

Includes

RNA purification filters, 2mL collection tubes, 1X PBS, RNA Purification Resuspension Solution, RNA Purification Wash Solution 1, RNA Purification Wash Solution 2, and Nucleic Acid Purification Elution Solution

This Applied Biosystems product is for the fast, easy, glass fiber filter-based isolation of RNA from blood using standard laboratory equipment in about one hour.

This Applied Biosystems product is for the fast, easy, glass fiber filter-based isolation of RNA from blood using standard laboratory equipment in about one hour. Contains sufficient reagents for RNA purification from 50 samples. Gene expression profiling and analysis of whole blood is becoming an increasingly important life science tool. Accurately measuring the molecular profile of blood-based RNA transcripts is a critical need in areas of research such as disease association studies, biomarker discovery and validation, and monitoring of drug treatment regimes.

  • Isolate high-quality RNA from blood, suitable for real-time PCR and microarray analysis
  • Obtain highly stable RNA virtually free of RNase activity, contaminating gDNA, or RT-PCR inhibitors
  • Easy workflow requires only standard laboratory centrifuges and ∼1 hour of time
  • No separate RBC lysis or proteinase K treatment required

Performance with Ease
The ability of the Tempus system (Tempus Blood RNA Tubes and Tempus Spin RNA Isolation Kit) to stabilize RNA expression profiles has been tested over a range of storage conditions. The combination provides stable RNA, high yields of purified RNA (observed yields of 6 to 25μg from a full blood draw from normal, healthy donors), and high-quality RNA (A260/280 ratio greater than 1.9 and RIN greater than 7.0) suitable for use in downstream applications such as quantitative PCR, microarray profiling, and other RNA analysis methods.

Accessory Product
The highly unstable nature of RNA transcripts ex vivo creates an even greater challenge for accurate expression analysis. Ongoing metabolic changes can drastically affect gene expression profiles in as little as one hour in standard blood collection tubes. Use of the Applied Biosystems Tempus™ Blood RNA Tubes (Cat. No. 4342792) is therefore recommended. These tubes are designed for direct collection of 3mL of blood into a plastic, evacuated blood collection tube containing RNA Stabilizing Reagent. The RNA, and therefore the expression profile, is stable in the collection tube for up to five days at room temperature and a minimum of 7 days at 4°C

Not high-throughput compatible (Manual)

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage • 50 RNA Purification Filters; room temperature
• 2 x 100 Collection Tubes; room temperature
• 2 x 80 mL 1X PBS; room temperature
• 24 mL RNA Purification Resuspension Solution; room temperature
• 2 x 22 mL RNA Purification Wash Solution 1; room temperature
• 120 mL RNA Purification Wash Solution 2; room temperature
• 4 x 1.9 mL Nucleic Acid Purification Elution Solution; room temperature

Isolation Technology Silica Spin Column
Purification Time 40 min.
Elution Volume 100 μL
Sample Type Whole Blood
Final Product Type Total RNA
High-throughput Compatibility Not High-throughput Compatible (Manual)
For Use With (Application) RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
Quantity 50 Preps
Shipping Condition Room Temperature
Starting Material Amount Up to 3 mL of blood
Yield ≤25 μg
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Your feedback has been submitted: Thank you for helping us improve our website.