Invitrogen™ Tempus™ Spin RNA Isolation Kit

Description

Includes

RNA purification filters, 2mL collection tubes, 1X PBS, RNA Purification Resuspension Solution, RNA Purification Wash Solution 1, RNA Purification Wash Solution 2, and Nucleic Acid Purification Elution Solution

This Applied Biosystems product is for the fast, easy, glass fiber filter-based isolation of RNA from blood using standard laboratory equipment in about one hour.

This Applied Biosystems product is for the fast, easy, glass fiber filter-based isolation of RNA from blood using standard laboratory equipment in about one hour. Contains sufficient reagents for RNA purification from 50 samples. Gene expression profiling and analysis of whole blood is becoming an increasingly important life science tool. Accurately measuring the molecular profile of blood-based RNA transcripts is a critical need in areas of research such as disease association studies, biomarker discovery and validation, and monitoring of drug treatment regimes.

• Isolate high-quality RNA from blood, suitable for real-time PCR and microarray analysis
• Obtain highly stable RNA virtually free of RNase activity, contaminating gDNA, or RT-PCR inhibitors
• Easy workflow requires only standard laboratory centrifuges and ∼1 hour of time
• No separate RBC lysis or proteinase K treatment required

Performance with Ease
The ability of the Tempus system (Tempus Blood RNA Tubes and Tempus Spin RNA Isolation Kit) to stabilize RNA expression profiles has been tested over a range of storage conditions. The combination provides stable RNA, high yields of purified RNA (observed yields of 6 to 25μg from a full blood draw from normal, healthy donors), and high-quality RNA (A260/280 ratio greater than 1.9 and RIN greater than 7.0) suitable for use in downstream applications such as quantitative PCR, microarray profiling, and other RNA analysis methods.

Accessory Product
The highly unstable nature of RNA transcripts ex vivo creates an even greater challenge for accurate expression analysis. Ongoing metabolic changes can drastically affect gene expression profiles in as little as one hour in standard blood collection tubes. Use of the Applied Biosystems Tempus™ Blood RNA Tubes (Cat. No. 4342792) is therefore recommended. These tubes are designed for direct collection of 3mL of blood into a plastic, evacuated blood collection tube containing RNA Stabilizing Reagent. The RNA, and therefore the expression profile, is stable in the collection tube for up to five days at room temperature and a minimum of 7 days at 4°C

Not high-throughput compatible (Manual)

Specifications

• Content And Storage: • 50 RNA Purification Filters; room temperature
  • 2 x 100 Collection Tubes; room temperature
  • 2 x 80 mL 1X PBS; room temperature
  • 24 mL RNA Purification Resuspension Solution; room temperature
  • 2 x 22 mL RNA Purification Wash Solution 1; room temperature
  • 120 mL RNA Purification Wash Solution 2; room temperature
  • 4 x 1.9 mL Nucleic Acid Purification Elution Solution; room temperature
  
  
• Isolation Technology: Silica Spin Column
• Purification Time: 40 min.
• Elution Volume: 100 μL
• Sample Type: Whole Blood
• Final Product Type: Total RNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
• Quantity: 50 Preps
• Shipping Condition: Room Temperature
• Starting Material Amount: Up to 3 mL of blood
• Yield: ≤25 μg

For Research Use Only. Not for use in diagnostic procedures.