Invitrogen™ TRIzol™ Plus RNA Purification Kit  

Isolate total RNA from animal and plant cells, tissue, bacteria, and yeast

Manufacturer:  Invitrogen™ 12183555

Catalog No. 12-183-555

Includes: TRIzol Reagent (100mL); Lysis Buffer (125mL); Wash Buffer I (50mL); Wash Buffer II (15mL); RNase-Free Water (15.5mL); Spin Cartridges with collection tubes (50 each); Collection Tubes (50 each); Recovery Tubes (50 each). Sufficient reagents are provided to perform 50 RNA isolations.

Description

The TRIzol Plus RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a wide variety of samples, including animal and plant cells, tissue, bacteria, and yeast. The kit combines the strong lysis capability of TRIzol Reagent, followed by a convenient and time-saving silica-cartridge purification protocol from the PureLink RNA Mini Kit, to provide ultrapure total RNA typically within an hour, even from difficult samples such as fibrous or fatty tissues.

Two products in one The TRIzol Plus RNA Purification System couples the lysis capabilities of TRIzol Reagent with the 1000 µg column-;binding capacity of the PureLink RNA Mini Kit, resulting in high RNA integrity and yield. The TRIzol Plus RNA Purification System includes protocols for a variety of sample types, including fibrous or fatty tissues and plant material. The combination of TRIzol Reagent followed by silica-cartridge purification of RNA is the recommended RNA purification method in the gene expression industry. The TRIzol Plus RNA Purification Kit provides the reagents and an optimized protocol to purify total RNA for gene expression studies using an industry recommended method.

Simple lysis to column purification protocol TRIzol Reagent sample lysis maintains the integrity of the RNA, while disrupting cells and dissolving cell components. TRIzol Reagent also provides an immediate and highly effective inhibition of RNase activity during sample homogenization. Following lysis, RNA in the lysed sample is bound to the clear silica-based membrane in the PureLink RNA Mini Kit Spin Cartridge, washed to remove contaminants, and then eluted in RNase-Free water (Tris Buffer, pH 7.5 may also be used).

• Combined TRIzol-based lysis and PureLink™ spin column-based RNA isolation technologies
• Superior lysis capability and convenient protocol
• Fewer resources used, less hazardous, less waste

Not high-throughput compatible (Manual)

DNA and RNA Purification and Analysis, Gene Expression Analysis and Genotyping, Microarray Analysis, RNA Extraction, RNA Isolation for Expression Microarrays, RNAi, Epigenetics and Non-Coding RNA Research, Total RNA Isolation, Total RNA from Animal Cells and Tissues, Total RNA from Bacterial Samples, Total RNA from Plant Cells, Total RNA from Yeast, miRNA and Non-Coding RNA Analysis, miRNA Isolation

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Order Info

Shipping Condition: Room temperature

Specifications

• Final Product Type: Total RNA
• Purification Target: Total RNA
• Format: Spin Column
• No. of Reactions: 50 Preps
• Yield: 1000 µg
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Scale: Mini
• System Type: TRIzol

• For Use With (Application): Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Nucleic Acid Labeling, Nuclease Protection Assays, Northern Blotting
• Sample Type: Plant Samples, Yeast, Tissue, Bacteria, Cells
• Isolation Technology: Silica Spin Column, Organic Extraction
• Quantity: 50
• Content And Storage: Kit Components:
  • TRIzol® Reagent (100 ml)
  • Lysis Buffer (125 ml)
  • Wash Buffer I (50 ml)
  • Wash Buffer II (15 ml)
  • RNase-Free Water (15.5 ml)
  • Spin Cartridges with collection tubes (50 each)
  • Collection Tubes (50 each)
  • Recovery Tubes (50 each)
  
  Store all components at room temperature. Sufficient reagents are provided to perform 50 RNA isolations.
• Purification Time: 20 min
• Starting Material Amount: Up to 5 x 10^8 cells, Up to 200 mg tissue
• Product Line: TRIzol®

For Research Use Only. Not for use in diagnostic procedures.