AMC (CAS No 26093-31-2) is a compound prevalent in biomedical research especially in the study of enzymatic activity Originating from the need to understand biological pathways AMC serves as a substrate in assays measuring enzyme kinetics and activity It is commonly used in studies involving serine proteases and other peptidases due to its ability to release a fluorescent signal upon enzymatic cleavage allowing researchers to monitor real-time enzyme activity Its effectiveness is often observed in nanomolar to low micromolar ranges making it suitable for high-throughput screening applications AMC is frequently applied in cell models and animal studies to evaluate enzyme inhibitors and explore potential therapeutic pathways Experimental concentrations typically depend on the specific research design highlighting its adaptability in various scientific investigations