Invitrogen™ Human AMPK alpha-1/beta-1/gamma-2, His Tag Recombinant Protein

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Recombinant full-length human AMPK (combination of A1/B1/G2 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. : Validated in LanthaScreen™ Kinase Binding Assay: Protocols available for simple use and optimized performance: Multiple pack sizes available for convenient use Related Product:LanthaScreen™ Eu Kinase Binding Assay Protein Form: Recombinant.

AMPK (A1/B1/G2) is a member of the AMPK family which are heterotrimeric proteins consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress. AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and hydroxy beta-methylglutaryl-CoA reductase. The beta subunit may be a positive regulator of AMPK activity and is highly expressed in skeletal muscle.

Specifications

• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM sodium phosphate with 0.2mM DTT, 25% glycerol, 150mM Imidazole, 300mM NaCl, 0.1mM PMSF and no preservative; pH 7
• Molecular Weight (g/mol): 63.6-38-65 kDa
• Name: Human AMPK alpha-1/beta-1/gamma-2, His Tag
• pH Range: 7
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Common Name: AMPK alpha-1/beta-1/gamma-2
• Protein Length: 10-end
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: 10-end
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Assay Entry: Biochemical binding/kinetic
• Kinase Group: Serine/Threonine Kinase
• Target Entry: AMPK (A1/B1/G2)
• Expression System: Baculovirus
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov