Invitrogen™ Human AMPK (A1/B1/G3), His Tag Recombinant Protein

Catalog No.	Quantity
PV6241	10 μg
PV6243	1 mg
PV6242	100 μg

Catalog No. PV6241 Supplier Invitrogen™ Supplier No. PV6241

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Recombinant full-length human AMPK (combination of A1/B1/G3 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. , Validated in LanthaScreen™ Kinase Binding Assay, Protocols available for simple use and optimized performance, Multiple pack sizes available for convenient use Protein Form: Recombinant The gene accession number for the three subunits (A1/B1/G3) are NP_006242.5, NP_006244.2, and NP_059127.2.

AMPK (A2/B2/G3) is a member of the AMPK family which are heterotrimeric proteins consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress. AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and hydroxy beta-methylglutaryl-CoA reductase. The beta subunit may be a positive regulator of AMPK activity and is highly expressed in skeletal muscle.

Specifications

• Accession Number: Q13131, Q9UGI9, Q9Y478
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM sodium phosphate with 0.2mM DTT, 25% glycerol, 150mM Imidazole, 300mM NaCl, 0.1mM PMSF and no preservative; pH 7
• Gene ID (Entrez): 53632, 5562, 5564
• Molecular Weight (g/mol): 63.6-38-51 kDa
• Name: Human AMPK (A1/B1/G3), His Tag
• pH Range: 7
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: AMPK alpha 1/beta 1/gamma 3; AMPK alpha1/beta1/gamma3
• Common Name: AMPK alpha-1/beta-1/gamma-3
• Gene Symbol: PRKAA1, PRKAB1, PRKAG3
• Protein Length: aa 10-end
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: 10-end
• Content And Storage: Storage Buffer: 50 mM sodium phosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol
  
  Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.
• Assay Entry: Biochemical binding/kinetic
• Kinase Group: Serine/Threonine Kinase
• Target Entry: AMPK (A1/B1/G3)
• Expression System: Baculovirus
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Fragment
• Form: Liquid
• Protein Subtype: Serine/Threonine Kinases
• Research Category: Drug Discovery: Signal Transduction