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Invitrogen™ Human AMPK alpha-1/beta-2/gamma-1, His Tag Recombinant Protein

Catalog No. PV6244
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PV6244 10 μg
PV6245 100 μg
PV6246 1 mg
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Catalog No. PV6244 Supplier Invitrogen™ Supplier No. PV6244
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Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Recombinant full-length human AMPK (combination of A1/B2/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. , Validated in LanthaScreen™ Kinase Binding Assay, Protocols available for simple use and optimized performance, Multiple pack sizes available for convenient use Related Product: LanthaScreen™ Eu Kinase Binding Assay Protein Form: Recombinant

AMPK (A1/B2/G1) is a member of the AMPK family which are heterotrimeric proteins consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress. AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and hydroxy beta-methylglutaryl-CoA reductase. The beta subunit may be a positive regulator of AMPK activity and is highly expressed in skeletal muscle.
TRUSTED_SUSTAINABILITY

Specifications

Concentration See Label
For Use With (Application) Kinase Assay
Formulation 50 mM sodium phosphate with 0.1 mM PMSF, 0.2 mM DTT, 150 mM Imidazole, 25% glycerol, 300 mM NaCl and no preservative; pH 7
Molecular Weight (g/mol) 63.6-36-40 kDa
Name Human AMPK alpha-1/beta-2/gamma-1, His Tag
Quantity 10 μg
Common Name AMPK alpha-1/beta-2/gamma-1
Protein Length 10-end
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Protein Tag His-tag
Content And Storage -80°C, Avoid Freeze/Thaw Cycles
Expression System Baculovirus
Form Liquid
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