Invitrogen™ Human AMPK alpha-2/beta-2/gamma-1, His Tag Recombinant Protein

Catalog No.	Quantity
PV6247	10 μg
PV6248	100 μg
PV6249	1 mg

Catalog No. PV6247 Supplier Invitrogen™ Supplier No. PV6247

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Recombinant full-length human AMPK (combination of A2/B2/G1 subunits) was expressed by baculovirus in Sf9 insect cells using C-terminal His tag. , Validated in LanthaScreen™ Kinase Binding Assay, Protocols available for simple use and optimized performance, Multiple pack sizes available for convenient use Related Product: LanthaScreen™ Eu Kinase Binding Assay Protein Form: Recombinant

AMPK (A2/B2/G1) is a member of the AMPK family which are heterotrimeric proteins consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress. AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and hydroxy beta-methylglutaryl-CoA reductase. The beta subunit may be a positive regulator of AMPK activity and is highly expressed in skeletal muscle.

Specifications

• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM sodium phosphate with 150mM Imidazole, 0.2mM DTT, 25% glycerol, 300mM NaCl, 0.1mM PMSF and no preservative; pH 7
• Molecular Weight (g/mol): 63.1-36-40 kDa
• Name: Human AMPK alpha-2/beta-2/gamma-1, His Tag
• pH Range: 7
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: Subunit A2: PRKAA2, AMPK, AMPK2, PRKAA Subunit B2: PRKAB2, MGC61468 Subunit G1: PRKAG1, AMPKG, MGC8666
• Common Name: AMPK alpha-2/beta-2/gamma-1
• Protein Length: 1-552, 1-272, 1-331
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: 1-552, 1-272, 1-331
• Content And Storage: Storage Buffer: 50 mM sodium phosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol
  
  Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.
• Assay Entry: Biochemical binding/kinetic
• Kinase Group: Serine/Threonine Kinase
• Target Entry: AMPK (A2/B2/G1)
• Expression System: Baculovirus
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Full Length
• Form: Liquid
• Protein Subtype: Serine/Threonine Kinases
• Research Category: Drug Discovery: Signal Transduction