Invitrogen™ Human Amyloid beta 40 ELISA Kit

Catalog No.	Quantity
KHB3482	2 x 96 Tests
KHB3481	96 Tests

Catalog No. KHB3482 Supplier Invitrogen™ Supplier No. KHB3482

Description

ELISA

The Human Amyloid Beta 40 (Hu Abeta 40) ELISA quantitates Hu Abeta 40 in human tissue culture medium, tissue homogenate, and cerebrospinal fluid (CSF). The assay will exclusively recognize both natural and recombinant Hu Abeta 40. Principle of the method The Human Abeta 40 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Beta-amyloid is a 40-43 amino acid peptide cleaved from amyloid precursor protein, and a putative gamma-secretase. Amyloid beta (1-40) together with Amyloid beta (1-42) are two major C-terminal variants of the amyloid beta protein, constituting the majority of amyloid beta proteins. Both the amyloid beta (40) and amyloid beta (42) alloforms are major components of the extracellular plaques found in Alzheimer's disease brain tissue.

Order Info

Shipping condition: Wet ice

Specifications

• Accession Number: P05067
• Assay Range: 7.8 to 500 pg/mL
• Assay Sensitivity: <6 pg/mL
• Concentration: 9.8 to 500 pg/mL
• Content And Storage: Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
• Conjugate: HRP
• Detection Method: Colorimetric
• Product Type: ELISA
• Sample Type: Cerebrospinal Fluid, Supernatant, Tissue Homogenates
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 351
• Gene Symbol: AAA,ABETA,ABPP,AD1I,CTFgamma,CVAP,PN-II,PN2,APP
• Incubation Time: 4 hr.
• Interassay CV: 3.8%
• Intraassay CV: 2.5
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Hu Aβ40 Detection Antibody, Anti-Rabbit IgG HRP (100 X), HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Marker Gene: Aβ 40
• Quantity: 2 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cerebrospinal Fluid 50 μL, Supernatant 50 μL, Tissue Homogenate 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Amyloid beta 40
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs

For Research Use Only. Not for use in diagnostic procedures.