Invitrogen™ Rat Androgen Receptor, Ligand Binding Domain, GST Tag, His Tag Recombinant Protein

Catalog No.	Quantity
A15675	2 mg

Catalog No. A15675 Supplier Invitrogen™ Supplier No. A15675

Description

Recombinant Protein

Androgen receptor ligand binding domain (AR-LBD) is a 64.42 kDa recombinant rat protein, expressed as a GST- and His-tagged protein in insect cells. The protein contains the hinge region and an LBD amino acid sequence identical to that of the human LBD. Differences in the rat and human hinge region are present at human amino acids 646, 649, 650, 655, 656, 657, and 660. AR-LBD is expressed and purified in the absence of exogenous ligands, making it ideal for ligand-binding and coregulator recruitment studies. AR-LBD rat recombinant protein is: Flexible—use in your in-house assay or as part of our PolarScreen™ Competitor assays for a complete assay solution Ready to use—active and tagged (with GST/His) Convenient—available in bulk sizes Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond. Protein Form: Ligand Binding Domain, Recombinant.

The androgen receptor (AR) is a member of the steroid-hormone receptor superfamily of nuclear receptors. The receptor is more than 90 kDa and has three major functional domains: the N-terminal domain, DNA-binding domain, and the androgen-binding domain. The androgen receptor is a ligand-activated transcription factor that binds active testosterone (T) and dihydrotestosterone (DHT). Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis.AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain.Androgen Receptor is a phosphoprotein, and also regulates mitogen-activated protein kinase (MAP kinase). The inhibition of the MEK1/2 pathway correlates directly with a change in phosphorylation state of the androgen receptor. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease, and male infertility. Mutations in this gene are associated with complete androgen insensitivity (CAIS).

Specifications

• Accession Number: P15207
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: Proprietary buffer with no preservative
• Gene ID (Entrez): 24208
• Name: Rat Androgen Receptor, Ligand Binding Domain, GST Tag, His Tag
• Purification Method: Purified
• Quantity: 2 mg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: AIS; Andr; androgen nuclear receptor variant 2; androgen receptor; androgen receptor (Testicular feminization), same as Tfm; androgen receptor splice variant 4b; Ar; AR8; AR-A; AW320017; DHTR; dihydrotestosterone receptor; HUMARA; HYSP1; KD; NR3C4; Nuclear receptor subfamily 3 group C member 4; RP11-383C12.1; SBMA; SMAX1; TFM; Variant AR45
• Common Name: Androgen Receptor
• Gene Symbol: AR
• Protein Length: 606-902
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Rat
• Recombinant: Recombinant
• Protein Tag: GST-tag, His-tag
• Sequence: 606-902
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Expression System: Baculovirus
• Protein Family: Growth Factors & Receptors
• Protein Form: Ligand Binding Domain, Recombinant
• Form: Liquid

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov