Invitrogen™ Mouse ANGPTL3 ELISA Kit

Catalog No.	Quantity
EMANGPTL3X5	5 x 96 Tests
EMANGPTL3	96 Tests
50-274-1200	10 x 96 Tests

Catalog No. EMANGPTL3X5 Supplier Invitrogen™ Supplier No. EMANGPTL3X5

Description

ELISA

The Mouse ANGPTL3 ELISA quantitates Ms ANGPTL3 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms ANGPTL3. Principle of the method The Mouse ANGPLT3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene encodes a member of a family of secreted proteins that function in angiogenesis. The encoded protein, which is expressed predominantly in the liver, is further processed into an N-terminal coiled-coil domain-containing chain and a C-terminal fibrinogen chain. The N-terminal chain is important for lipid metabolism, while the C-terminal chain may be involved in angiogenesis. Mutations in this gene cause familial hypobetalipoproteinemia type 2.

Specifications

• Accession Number: Q9R182
• Assay Range: 40.96-10000 pg/mL
• Assay Sensitivity: 40 pg/mL
• Calibration Range: 40 pg/mL to 10,000 pg/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Proteases Peptidases & Inhibitors
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse 6 Ckine, Activin A, ADAMTS1, Adiponectin, Angiopoietin-3, ANGTL-4 Artemin, CCL28, CD36, Chordin, CRP, E-Cadherin, Epigen, Epiregulin, Fas, Galectin-7, gp130, Granzyme B, Gremlin, IFN-R1, IL-17 B, IL-17 B R, IL-22, MIP-1 b, MMP-2, MMP-3, MMP-10, PDGF-AA, Persephin, sFRP-3, Shh-N, SLAM, TCK-1, TECK, TGFb1, TNF RI, TNF RII, TPO, TRANCE, TremL1, TWEAK, VEGF-B, VEGF-R2, VCAM-1, VEGF.
• Sufficient For: 5 x 96 Assays
• Target Species: Mouse
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: ANG-5; angiopoietin 5; angiopoietin like 3; Angiopoietin-5; angiopoietin-like 3; angiopoietin-like protein 3; Angiopoietin-related protein 3; ANGPT5; ANGPTL3; ANGPTL3(17-221); ANGPTL3(17-224); ANL3; FHBL2; hypl; hypolipidemia; UNQ153/PRO179ANG-5; angiopoietin 5; angiopoietin like 3; Angiopoietin-5; angiopoietin-like 3; angiopoietin-like protein 3; Angiopoietin-related protein 3; ANGPT5; ANGPTL3; ANGPTL3(17-221); ANGPTL3(17-224); ANL3; FHBL2; hypl; hypolipidemia; UNQ153/PRO179; hypl
• Gene ID (Entrez): 30924
• Gene Symbol: ANGPTL3
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: Angiopoietin-like protein 3
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Endocrinology
• Sample Volume: Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Synonym: RP23-101O16.2, hypl
• Target: ANGPTL3
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min