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STEMCELL Technologies Annexin V, PE, Size: 25 Tests
Supplier: STEMCELL Technologies 1000330
Annexin V is a member of the annexin family of proteins that bind to membrane phospholipids in the presence of calcium. This dye has high affinity for phosphatidylserine (PS) that is present in the inner leaflet of the plasma membrane. Verified for use with flow cytometry assays use this antibody to detect the translocation of PS to the external environment. Annexin V is used along with viability dyes such as 7-AAD (Catalog 75001) or Propidium Iodide (Catalog 75002). The process of PS translocation occurs prior to the loss of membrane integrity. Therefore as cells progress through apoptosis and towards necrosis the cell membrane is compromised and consequently viability dyes pass into the cell. Thus cells undergoing early apoptosis stain positive for Annexin V and negative for viability dyes while apoptotic death or necrosis is characterized by positive staining for both Annexin V and the viability d
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