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Aves Labs, Inc Anti-Green Fluorescent Protein (GFP) Antibody
Supplier: Aves Labs, Inc GFP1020
Chickens were immunized with recombinant GFP protein. After repeated injections, immune eggs were collected from the hens, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using an agarose matrix to which the recombinant protein was attached. The final preparation of this product involved mixing affinity purified anti-GFP antibody (final concentration of 25 ug/ml) with IgY fractions of anti-GFP antibody (final concentration of 10 mg/ml) in PBS with 50% glycerol. This preparation was then filter-sterilized. Chicken anti-GFP (Green Fluorescent Protein) antibodies, 0.4 ml, 10.0 mg/ml. Recommended Dilutions: 1:1000 Immunohistochemistry, 1:2500-1:5000 Western Blot.
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