This ELISA kit detects AECA (anti-endothelial cell antibodies) in human serum samples using a qualitative indirect ELISA method Designed for research and diagnostic applications it provides reliable results within 1 to 5 hours requiring only 50-100 L of serum per test The assay utilizes a standardized protocol where AECA binding is measured at 450 nm ensuring specificity and sensitivity for detecting antibodies targeting endothelial cell antigens Ideal for studying autoimmune diseases vascular disorders or transplant-related complications this kit offers a streamlined workflow with clear procedural guidelines Its qualitative design focuses on confirming the presence or absence of AECA making it a practical tool for laboratories prioritizing efficiency in antibody screening without quantitative analysis requirements