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ECM Bioscience N-Cadherin (Y860)[E-Cadherin (Y835)], phospho-specific Rabbit Polyclonal Antibody, ECM Biosciences

Supplier:  ECM Bioscience CP1951

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This antibody was cross-adsorbed to phospho-N-Cadherin (Tyr-820) and unphosphorylated N-cadherin (Tyr-860) peptides before affinity purification using phospho-N-cadherin (Tyr-860) peptide. In western blots, the antibody detects a 130 kDa* band corresponding to N-cadherin (Tyr-860) in human endothelial cells treated with pervanadate and detects a 120 kDa band corresponding to E-cadherin (Tyr-835) in human A431 cells treated with pervanadate. The immunogen was Phospho-N-Cadherin (Tyr-860) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding tyrosine 860 in human N-cadherin. This sequence is conserved in human E-cadherin (Tyr-835), P-cadherin (Tyr-782), R-cadherin (Tyr-870), and has significant homology to VE-Cadherin (Tyr-733). Accession #P19022. Applications = WB, E, ICC; Species Reactivity = Hu, Rt, Ms; Size = 100 μl; Buffer = PBS, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year. Other Names = Cdh2, CD325

Catalog No. 50-206-8679


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