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ECM Bioscience p130 Cas (Tyr-762), phospho-specific Rabbit Polyclonal Antibody, ECM Biosciences
Supplier: ECM Bioscience PP1451
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-p130 Cas (Tyr-751) peptide before affinity purification using phospho-p130 Cas (Tyr-762) peptide (without carrier). The antibody detects a 130 kDa* protein corresponding to the molecular mass of phosphorylated p130 Cas on SDS-PAGE immunoblots of pervanadate treated human A431 cells, but not in control cells. The immunogen was Phospho-p130 Cas (Tyr-762) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 762 in the Src-binding domain of rat p130 Cas. This rat sequence is the same as Tyr-664 in human p130 Cas and Tyr-668 in mouse p130 Cas. This site is also highly conserved in Cas-L (HEF1, Nedd9). Accession #Q63767. Applications = WB, E; Species Reactivity = Hu, Rt, Ms; Size = 100 μl; Buffer = PBS, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year. Other Names = BCAR1, Cas
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