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Rockland Immunochemicals Fluorescent TrueBlot: Anti-Rabbit IgG DyLight™ 800 - 18-4516-32
Supplier: Rockland Immunochemicals 18451632
Fluorescent Rabbit TrueBlot Antibody DyLight™ 800 may also be used for detection in immunoassays that do not employ IP. Provided as a lyophilized powder. We recommend incubating the blots from minigels in sealed bags with minimal volume (2-3 mLs). If used conservatively at 2.5mLs/blot will yield enough reagent for 200 blots. Note that there are three key procedural considerations: 1. Protein A or G should not be used for the IP. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. For IP, Anti-rat IgG beads, or Anti-rabbit IgG beads should be used for rat or rabbit immunoprecipitating antibodies, respectively. 2. Immunoprecipitate should be completely reduced. 3. Bovine Serum Albumin, or MB-070 Blocking Buffer for Fluorescent WB, at low concentrations, should be used as the blocking protein for the WB. DO NOT USE BLOTTO or MILK. S
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