Antibody;1MG;Mouse IgG1/kappa;The cross-reactivity with other coronaviruses has not been tested yet.;Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb (NUN-S47) was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant SARS-CoV-2 Nucleocapsid protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
It is recommended to use antibody (NUN-S47) and (NUN-S46) as a pair set to detect SARS-CoV-2 Nucleocapsid in ELISA or serological assays.; the protein migrates as 25 kDa and 55 kDa under reducing (R) condition, and 130 kDa under non-reducing (NR) condition (SDS-PAGE).;Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C5227) at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse mAb (Cat. No. NUN-S47) with a linear range of 0.15-2.5 ng/mL (QC tested).