Thermo Scientific™ Antibody Purification Bundle

Description

Includes

Pierce Protein A/G Magnetic Agarose Beads (1 mL), Pierce IgG Elution buffer, pH 2.0 (1 L), Pierce Protein A/G IgG Binding Buffer (240 mL)

Thermo Scientific Antibody Purification Bundle combines optimized reagents to purify antibodies from serum, cell culture supernatant, or ascites. The bundle includes Thermo Scientific Pierce Protein A/G Magnetic Agarose beads, Pierce IgG binding and Elution Buffers

• Our chimeric Protein A/G binds to all human IgG subclasses, making it the ideal choice for purification of polyclonal or monoclonal IgG antibodies whose subclass identities have not been determined. In addition, it binds to IgA, IgE, IgM and (to a lesser extent) IgD. Protein A/G binds well to all mouse IgG subclasses but does not bind mouse IgA, IgM, or serum albumin. This makes Protein A/G an excellent tool for purification and detection of mouse monoclonal antibodies from IgG subclasses, without interference from IgA, IgM, and murine serum albumin
• Pierce IgG Binding and Elution Buffers enable high-yield, non-denaturing antibody purification. Nonspecifically bound serum or host cell protein can be washed away before dissociating bound antibody with the included optimized Pierce A/G IgG Binding buffer. The Protein A/G IgG Binding Buffer is a phosphate-based buffer containing EDTA. Pierce IgG Elution Buffer enables high-yield, non-denaturing antibody purification through efficient dissociation and elution of antibodies

Specifications

• Quantity: 1 Bundle
• Includes: Pierce Protein A/G Magnetic Agarose Beads (1 mL), Pierce IgG Elution buffer, pH 2.0 (1 L), Pierce Protein A/G IgG Binding Buffer (240 mL)
• Content And Storage: See instructions for individual components
• Column Type: Affinity Column