This kit detects KI-67 in human serum plasma and tissue homogenates using a sandwich ELISA methodology providing quantitative results within a detection range of 0 312 ng/mL to 20 ng/mL With a sensitivity of 0 078 ng/mL it enables precise measurement of low-abundance KI-67 antigen making it suitable for research applications requiring assessment of cellular proliferation markers The assay requires 50-100 L of sample per test and can be completed in 1-5 hours featuring a straightforward workflow optimized for laboratory efficiency Absorbance measurements at 450 nm ensure reliable data interpretation while the standardized protocol minimizes inter-experimental variability This kit is validated for reproducibility and specificity offering researchers a robust tool to study KI-67 expression in various biological matrices