Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. 503107255

Apexbio Technology LLC Annexin V-Biotin/PI Apoptosis Kit 200 assays
SDP

Catalog No. 503107255
Encompass_Preferred

Annexin V is a Ca2 dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS resides on the inner surface of the cell membrane s phospholipid bilayer During early stages of apoptosis it translocates from the inner leaflet to the outer surface thus exposing itself to the extracellular environment Biotin labeled Annexin V can be detected through binding with fluorescently labeled streptavidin (such as fluorescein-conjugated streptavidin or PE-conjugated streptavidin) The occurrence of apoptosis can then be identified using flow cytometry or fluorescence microscopy within only 10 to 20 minutes PI (propidium iodide) binds specifically to double-stranded DNA producing a red fluorescence signal PI cannot penetrate the intact cell membrane but late apoptotic and dead cells staining their nuclei Simultaneous staining with Annexin V-Biotin and PI permits differentiation among cells at various stages of apoptosis and dead cells

Catalog No. 50-310-7255 Supplier Apexbio Technology LLC Supplier No. K2009200
May include imposed supplier surcharges.
Add to Cart
Add to Cart

Annexin V is a Ca2 dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS resides on the inner surface of the cell membrane s phospholipid bilayer During early stages of apoptosis it translocates from the inner leaflet to the outer surface thus exposing itself to the extracellular environment Biotin labeled Annexin V can be detected through binding with fluorescently labeled streptavidin (such as fluorescein-conjugated streptavidin or PE-conjugated streptavidin) The occurrence of apoptosis can then be identified using flow cytometry or fluorescence microscopy within only 10 to 20 minutes PI (propidium iodide) binds specifically to double-stranded DNA producing a red fluorescence signal PI cannot penetrate the intact cell membrane but late apoptotic and dead cells staining their nuclei Simultaneous staining with Annexin V-Biotin and PI permits differentiation among cells at various stages of apoptosis and dead cells

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.