Apexbio Technology LLC Annexin V-Cy3/SYTOX Green Apoptosis Kit 100assays

Supplier:  Apexbio Technology LLC K114050

Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane lipid bilayer but flips from the inner to the outer leaflet during the early stages of apoptosis becoming exposed to the extracellular environment Annexin V has a high affinity for PS By conjugating Annexin V with Cy3 the labeled Annexin V serves as a fluorescent probe enabling detection of apoptosis events via flow cytometry or fluorescence microscopy within only 10 20 minutes staining SYTOX Green is a membrane-impermeable cyanine dye that normally cannot penetrate intact cell membranes However during late-stage apoptosis or necrosis SYTOX Green can cross disrupted regions of the cell membrane reach the nucleus and bind DNA double helix molecules forming SYTOX Green-DNA complexes Binding increases fluorescence intensity over 500-fold generating bright green fluorescence Thus SYTOX Green stains o

Catalog No. 50-310-7289