Apexbio Technology LLC Annexin V-Cy5/DAPI Apoptosis Kit 20assays

Supplier:  Apexbio Technology LLC K2255100

Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (Cy5) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes DAPI specifically binds to double-stranded DNA emitting blue fluorescence but normally cannot permeate intact cell membranes However DAPI can enter late-stage apoptotic or dead cells and stain their nuclei Simultaneous staining with Annexin V-Cy5 and DAPI allows differentiation of cells at various stages of apoptosis and cell death

Catalog No. 50-310-7172