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Apexbio Technology LLC Annexin V-PE/7-AAD Apoptosis Kit 200tests
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Catalog No. 503107092
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Annexin V-PE/7-AAD Apoptosis Kit enables differentiation between early apoptotic late apoptotic and dead cells Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane phospholipid bilayer However during the early stages of apoptosis PS moves from the inner surface to the outer surface of the cell membrane becoming exposed to the extracellular environment Labeled with a fluorescence dye such as PE Annexcin V is commonly used as a fluorescent probe to detect apoptosis through flow cytometry or fluorescence microscopy within 15-30 minutes In addition this kit includes 7-AAD a nucleic acid dye that specifically binds double-stranded DNA and emits red fluorescence Normally 7-AAD cannot permeate intact cell membranes however it can penetrate compromised membranes of late apoptotic and dead cells staining their nuclei

Catalog No. 50-310-7092 Supplier Apexbio Technology LLC Supplier No. K2283200
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Annexin V-PE/7-AAD Apoptosis Kit enables differentiation between early apoptotic late apoptotic and dead cells Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane phospholipid bilayer However during the early stages of apoptosis PS moves from the inner surface to the outer surface of the cell membrane becoming exposed to the extracellular environment Labeled with a fluorescence dye such as PE Annexcin V is commonly used as a fluorescent probe to detect apoptosis through flow cytometry or fluorescence microscopy within 15-30 minutes In addition this kit includes 7-AAD a nucleic acid dye that specifically binds double-stranded DNA and emits red fluorescence Normally 7-AAD cannot permeate intact cell membranes however it can penetrate compromised membranes of late apoptotic and dead cells staining their nuclei

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