Apexbio Technology LLC AO/PI Double Staining Kit 500T

Supplier:  Apexbio Technology LLC K2238500

The AO/PI Double Staining Kit is a reagent kit enabling rapid detection of cell viability This kit provides two nuclear dyes Acridine Orange (AO) and Propidium Iodide (PI) AO can penetrate intact cell membranes and stain viable cells green During apoptosis chromosomes become condensed or fragmented into differently sized segments In this scenario AO stains apoptotic cells densely with yellow-green fluorescence or as yellow-green fragments The other nuclear dye PI cannot pass through intact cell membranes therefore it does not stain viable or apoptotic cells in red but stains cells with compromised membranes (e g necrotic cells) When using these two dyes in combination viable cells exhibit green fluorescence apoptotic cells show dense varied-size yellow-green or orange fluorescence and necrotic cells exhibit strong red fluorescence

Catalog No. 50-310-7295