Human Cardiac-Related Fibroblasts include Aortic Adventitial Fibroblasts (AoAF) isolated from the tunica external of the ascending or descending aorta and cardiac fibroblasts (NHCF-A and -V) isolated from the atrium or ventricle Cells are isolated from normal adult heart tissue Cryopreserved cells are shipped at passage 2 Clonetics NHCF-V -A are guaranteed through 5 population doublings and express 90% collagen I and 10% Von Willibrand factor VIII after thaw Clonetics AoAF are guaranteed through 10 population doublings and are negative for alpha smooth muscle actin All cells test negative for mycoplasma bacteria yeast and fungi HIV-1 hepatitis B and hepatitis C are not detected for all donors and/or cell lots A Certificate of Analysis (CoA) is provided for each cell lot purchased